Relationship Between Thomsen-Friedenreich (TF) Antigen and Anti-tumor Vaccine

TF antigen is an oligosaccharide structure containing galactose and N-acetylcysteine. Because of the high level of expression and specificity in tumors, the study of TF antigens is important for the development of antitumor vaccines. For example, TF antigen as a targeting molecule for antitumor vaccines allows the recognition and attack of TF antigen-expressing tumor cells by stimulating the immune system of the body to generate an immune response against the TF antigen. This way can provoke the defense mechanism of the body to remove tumor cells, which is better safe and controllable. Moreover, the study of the application of TF antigen in antitumor vaccines simultaneously promotes the researchers' further understanding of the immune escape mechanism and immune tolerance mechanism of tumor cells, which contributes to further research.

Fig.1 Osawa and Kaifu's synthesis of the TF antigen from their previously prepared Tn antigen. (Sadraei, et al., 2017)

TF Antigen Production Service at CD BioGlyco

CD BioGlyco provides professional Tumor-Associated Glycopeptide Antigen Production Services, including TF antigen production service, to our clients with advanced Glyco™ Vaccine Development Platform and efficient technologies. Our services include but are not limited to chemical synthesis, enzymatic synthesis, and large-scale isolation and purification processes:

• Chemical synthesis

We gradually synthesize and precisely control the glycan chain structure of TF antigens using organic synthetic chemistry after customizing the synthesis steps and synthesis strategy to your needs.

• Enzymatic synthesis

We synthesize TF antigens in vitro using specific enzymes or enzyme combinations such as glycosyltransferases or glycosidases that mimic the synthesis process in living organisms.

• Extraction and purification method

We extract a mixture of glycan chains or proteins containing the TF antigen from a biological sample and purify them by chromatography and other methods. This allows direct access to TF antigen in its natural state.

Fig.2 Process for the enzymatic synthesis of TF antigen. (CD BioGlyco)

Gastric cancer is one of the cancers associated with high expression of TF antigen. We also offer the development of TF antigen-based vaccines for gastric cancer. We combine TF antigen with appropriate vectors to enhance its immunogenicity and activate the immune response capacity of the immune system of the body.

Applications

• Multiple pathways can be used to apply TF antigens in anti-tumor vaccine development, providing new strategies and methods for antitumor vaccine research.
• By studying TF antigen in-depth, researchers can better understand the role it plays in disease progression and target it for drug design and development.

Advantages of Us

• Our TF antigen production method ensures consistency and stability through standardized operating procedures and controlled conditions.
• We personalize and fine-tune comprehensive and stringent experimental protocols to precisely align with the scientific requirements of our clients.
• We integrate antibody production strategies with diverse detection technologies to optimize production efficiency.

By utilizing our versatile glyco vaccine development platform, CD BioGlyco employs a diverse range of methods to provide TF antigen production. Our team selects the most suitable synthesis method tailored to your needs. Please feel free to contact us for further details.

Reference

1. Sadraei, S.I.; et al. The synthesis and biological characterization of acetal-free mimics of the tumor-associated carbohydrate antigens. Advances in Carbohydrate Chemistry and Biochemistry. 2017, 74: 137-237.