Tumor-associated glycopeptide antigens are a specific class of antigens that are highly expressed on the surface of tumor cells, while anti-tumor vaccines are a way to fight tumors by stimulating the immune system. Anti-tumor vaccines can use tumor-associated glycopeptide antigens as immunogens to direct the immune system of the body to recognize and attack tumor cells. With our state-of-the-art Glyco™ Vaccine Development Platform, CD BioGlyco offers a seamless route to your R&D regarding tumor-associated glycopeptide antigen production. Our antigen production services include but are not limited to the following.
In addition to providing glycopeptide antigen production services, we also provide subsequent glycopeptide antigen vaccine development services. We provide you with immunogenicity assessment and help to select and construct suitable vaccine vectors based on the assessment results. By preparing different types of glycopeptide antigens, vaccines can be developed against different diseases, such as cancer and infectious diseases.
Paper Title: O-glycosyl α-amino acids as building blocks for glycopeptide synthesis
Journal: Tetrahedron: Asymmetry.
Abstract: This article is a comprehensive overview of O-glycosylated α-amino acid structures, encompassing the majority of glycosylation sites found in naturally occurring O-glycoproteins. Chemical synthesis methods for solution or solid-phase synthesis of these structures are also discussed.
Method: For stepwise solid-phase peptide synthesis, which is usually based on the Fmoc strategy, it is crucial to prepare appropriately protected glycosyl amino acid building blocks. To achieve mild deprotection of the assembled glycopeptide, both the carbohydrate and amino acid functional groups must utilize a distinct set of orthogonal protecting groups. Then the temporary amine protection of the peptide chain, conferred by the Fmoc group, can be selectively removed through meticulous treatment with morpholine or piperidine. Under acidic conditions, the O-glycan bond exhibits instability. To ensure the stability of the acetal bond, it is recommended to employ O- and N-acetyl groups as protective measures for the glycan component.
Fig.1 Structures of TN antigen, TF antigen, and sTN antigen. (Arsequell & Valencia, 1997)
Detection methods for tumor-associated glycopeptide antigens include immunoassays (e.g., ELISA), immunohistochemistry (IHC), immunoflow cytometry (FACS), mass spectrometry, and biosensor technology. These methods can accurately and rapidly detect the presence and expression level of glycopeptide antigens and provide important tools for tumor research.
CD BioGlyco has earned the unwavering trust of scientists worldwide through our exceptional high-quality tumor-associated glycopeptide antigen production service. If you are interested in our services, we warmly invite you to contact us for further comprehensive information.
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