Principle of Thermogelation Technology

The principle of thermogelation technology is to take advantage of the low critical solubility temperature (LCST) property of thermosensitive polymers. At temperatures below LCST, the thermosol remains in liquid form, while at temperatures above LCST, the thermosol forms a gel through a gelation process. By controlling the temperature change, repeated reversible transitions between liquid and gel states can be achieved. In thermogelation technology, a specific recognition ligand for the target molecule is usually added to the thermosensitive polymer solution to make it bind specifically to the target molecule. Polysaccharide conjugation reaction based on thermal gelation technology refers to the conjugation of polysaccharides with other molecules, proteins, or drugs, through the process of thermal gelation for applications such as modification, functionalization, or drug delivery of polysaccharides. The principle is mainly through a combination of hydrophobic and electrostatic interactions, where substances with hydrophobic or charged amounts interact with polysaccharides to achieve polysaccharide conjugation.

Thermogelation-based Polysaccharide Conjugation Services at CD BioGlyco

CD BioGlyco offers  the right support and specialized knowledge in the development of your glycobiology program. We design different procedures to provide customized polysaccharide conjugation services based on diverse Technologies according to the scientific needs of our clients, including the thermogelation method. The specific services are as follows:

• Polysaccharide conjugation services based on electrostatic interactions
  Formation of electrostatic conjugated structures through conjugation reactions of polysaccharides with oppositely charged molecules. The conjugated structure formed is discrete and the molecules are widely spaced, making it suitable for your molecules with high requirements for reversibility and modifiability.
• Polysaccharide conjugation services based on hydrophobic interactions
  Polysaccharides as a hydrophobic core are conjugated with other hydrophilic molecules through hydrophobic interactions. This thermogelation system provides high stability and forms stable gel states in solvents. The high structural stability of these gel systems is commonly used in materials science and drug delivery.

Fig.1 Flowchart of polysaccharide conjugation based on electrostatic interactions. (CD BioGlyco)

Applications

• Development of new glycolic drugs
• Research on immune response
• Improving the specificity of polysaccharides

Advantages of Us

• High-temperature sensitivity
• Conjugate processes that are regulated as needed
• Customized one-stop services

CD BioGlyco utilizes the Glyco™ Vaccine Development Platform to offer Polysaccharide Conjugation services for clients based on thermogelation. Our professional team will precisely control the reaction process and use various analytical methods to monitor and optimize the reaction to ensure the stability and functionality of the conjugation. If you are interested in our services, please contact us for more details.

Reference:

1. Haque, A.; et al. Thermogelation of methylcellulose. Part I: molecular structures and processes. Carbohydrate polymers. 1993, 22(3): 161-173.