Different Kinds of Oligo Amino Linkers

Amino linker modification of oligonucleotides is common. The primary amino groups can further link various functional modification reagents to oligonucleotides, or attach oligonucleotides to solid surfaces for use as DNA probes or in purification experiments. There are three kinds of amin-modified oligonucleotides: 5'-amino, internal amino, and 3'-amino linkers.

• 5'-amino linker modification: The oligo amino linker is mainly used to prepare functional oligonucleotides to realize the assembly of DNA chips and the establishment of a multi-label diagnostic system.
• Internal amino linker modification: The amino linkers are added to the base or the ribose of oligonucleotides, and the assembled molecules can be further labeled or attached to enzymes (such as alkaline phosphatase).
• 3'-amino linker modification: The modification can be applied to make various diagnostic probes. When the 5' end is attached to ³²P with high sensitivity or fluorescein, the 3' end can be linked to functional other groups by the amino linker. It is also used to prepare antisense nucleotides. When the 3 'end of the oligonucleotide is linked by an amino linker, the enzymolysis of the 3'→5' exonuclease loses its efficacy, which can be used in antisense experiments.

Fig.1 Amino oligo conjugation to NHS ester ligand. (CD BioGlyco)

Amino-based Oligonucleotide Modification Service at CD BioGlyco

CD BioGlyco is a leading company specializing in Sugar-nucleotides Synthesis. We focus on the exploitation of new technologies in Oligonucleotide Synthesis and Modification as well as the production of nucleotides. We have developed various amino-based oligonucleotide modification techniques.

• 5'-Amino linkers
  The amino group is added to the 5' end of the oligonucleotide, and the alkane chain containing 3, 6, or 12 carbon atoms (C3, C6, C12) is used as a hydrophobic spacer to reduce the steric hindrance. By the reaction of NH₂ groups and activated ester groups to form stable amide bonds, CD BioGlyco functionalizes the oligonucleotides according to your requirements.

Fig.2 Examples of 5'-amino linkers. (CD BioGlyco)

• Internal amino linkers
  
  • 2'-amino linkers: Amino modification at the ribose 2' position can enhance the base pairing of the oligonucleotide to the target, improve the stability of the double strand, and enhance its specificity and resistance to nucleases. It is a useful method to design antisense oligonucleotides and aptamers.
  • Amino linker on the base: We provide amino linker modification services with different spacers in the base portion of the oligonucleotide, including amino-C6-dA, amino-C6-dC, amino-C6-dG, amino-C6-dT, and amino-C2-dT. This will enable you to further incorporate other functional groups such as biotin, quencher, fluorescent dye, etc. into the oligonucleotide fragments.

Fig.3 Some oligonucleotides with internal amino linkers. (CD BioGlyco)

• 3'-amino linkers: Oligonucleotides with 3'-end-modification are resistant to 3'→5' exonuclease and can be used as 3'-end blockers and highly effective polymerase chain terminators. In addition, with a 3'-amino linker, you can further add the functional groups you expect on the oligonucleotide, as with a 5'-amino linker. We offer various types of 3'-amino linker modification, including, C3, C6, and C7 amino linkers.

Fig.2 Examples 3'-amino linkers. (CD BioGlyco)

Applications

• Preparation of oligonucleotides modified by functional groups.
• Preparation of functional probes.
• Preparation of DNA chips.
• Preparation of specific purification column.
• Production of antisense oligonucleotides and aptamers.

Highlights

• Superb synthesis technology.
• Various amino linker modifications.
• Customized functionalized oligonucleotide synthesis service.

Since its establishment, CD BioGlyco has been continuously providing high-quality oligonucleotides for global clients and successfully applied them in various fields of molecular biology. If you are interested in our Platforms and would like more information, please feel free to contact us immediately and we will get back to you as soon as possible.