Custom Sugar-Nucleotide Synthesis

Sugar-nucleotide is of great significance in mediating biological processes and the development of carbohydrate drugs. CD BioGlyco has many years of experience in sugar-nucleotide synthesis and can customize high-quality synthesis services according to customers' research needs. We have the confidence to be your essential research assistant in the field of glycobiology.

The Structure of Sugar-Nucleotide

Structurally, a sugar-nucleotide is composed of a sugar or sugar derivative and a nucleoside monophosphate or diphosphate. Sugar-nucleotide is a necessary intermediate for carbohydrate metabolism and glycoconjugate biosynthesis. The biosynthesis of glycans and glycoconjugates depends on the activation of monosaccharides as sugar nucleotides. They are composed of an activated sugar donor that is glycosylated onto a diverse range of acceptors, typified by glycosyltransferase-catalyzed processes for the assembly of glycosides and oligo- or polysaccharides.

Sugar-nucleotides and structural analogs can be used as substrates for enzymatic reactions in carbohydrate synthesis, as powerful tools for studying glycoconjugate biosynthesis, and they have the potential as enzyme inhibitors in new therapeutic strategies. Besides, they are also important for the provision of structurally defined homogenous analytical standards.

Therefore, the effective preparation of natural and unnatural sugar-nucleotide is of great significance in both synthetic biology and medicinal chemistry. The synthesis of sugar-nucleotides is more difficult because of many factors: Sugar-nucleotides synthesis can be difficult due to many factors: the low solubility of sugar nucleotides in organic solvents, the presence of polar or charged functional groups, as well as the sensitivity of glycosidic and pyrophosphate bonds to hydrolytic cleavage.

Fig 1. Representative examples of common mammalian sugar-nucleotides. (Ahmadipour, et al., 2018)

Custom Sugar-Nucleotides Synthesis at CD BioGlyco

CD BioGlyco provides a variety of professional sugar-nucleotide synthesis services. Clients have several options to choose what service suits their research plan the most. Besides the custom sugar-nucleotides synthesis services we offer, CD BioGlyco also provides other Custom Carbohydrate Synthesis Services on our Glyco™ Synthesis Platform.

Chemical synthesis can prepare various natural and unnatural structures. This method has multiple steps and is challenging.
Enzymatic synthesis has the characteristics of high location specificity and stereospecificity. It simulates the synthesis steps of sugar chains in organisms and is an effective alternative to organic chemical synthesis.
Combining chemical and enzymatic methods. Firstly, the core precursor is prepared by chemical synthesis, and then the precursor is expanded into a variety of complex glycans by glycosyltransferase catalysis.

CD BioGlyco also offers specialized services for One-pot Multienzyme (OPME)-based NDP-sugar Synthesis and One-pot Multienzyme (OPME)-based NMP-sugar Synthesis. These services enable the efficient synthesis of NDP-sugars and NMP-sugars, which are crucial intermediates in glycosylation processes.

Nucleoside & Nucleotide Synthesis Service

Nucleoside & Nucleotide Synthesis crafts DNA/RNA components via chemical or enzymatic methods, crucial for genetics, diagnostics, and therapeutics.

Custom Oligonucleotide Synthesis Service

Custom oligonucleotide synthesis tailors DNA/RNA sequences, enabling precise genetic research and therapeutic development.

Polynucleotide Synthesis Service

Polynucleotide synthesis forms DNA/RNA chains through sequential addition of nucleotides, vital for genetic information transfer and cellular functions.

Nucleoside & Nucleotide Modification Service

Nucleoside & Nucleotide modification alters DNA/RNA components, enhancing stability, binding, and function for diverse applications in biotechnology and medicine.

Oligonucleotide Modification Service

Oligonucleotide modification involves altering DNA/RNA sequences for improved properties, impacting diagnostics and molecular studies across disciplines.

Nucleoside-based Production Service

Nucleoside production involves creating the fundamental building blocks of DNA and RNA, comprising a nitrogenous base and a sugar molecule.

Nucleotide-based Production Service

Nucleotides are composed of three main components: a nitrogenous base, a sugar molecule (ribose or deoxyribose), and one or more phosphate groups.

Applications of Custom Sugar-Nucleotide Synthesis

Development of tools to study the biosynthesis of carbohydrates and glycoconjugates.
Enzyme inhibitor development.
Carbohydrate structure and function research.

Advantages of Us

Able to synthesize a variety of nucleotide sugars.
Outstanding process development, optimization, and scale-up.
Customized route design.
Selection of the appropriate counter ion to the phosphate group in the molecule to preserve the high purity.

CD BioGlyco customizes different experimental programs to meet the different research needs of customers. We have first-class experimental equipment and experienced researchers to provide you with professional sugar-nucleotide synthesis services. Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please feel free to contact us for more detailed information.

References

Ahmadipour, S.; et al. Recent advances in the enzymatic synthesis of sugar-nucleotides using nucleotidyltransferases and glycosyltransferases. Carbohydrate Research. 2018.
Feng, C.; et al. A dual-enzyme-assisted three-dimensional DNA walking machine using T4 polynucleotide kinase as activators and application in polynucleotide kinase assays. Analytical chemistry. 2018, 90(4): 2810-2815.
Baroud, M.; et al. The evolution of nucleosidic analogues: self-assembly of prodrugs into nanoparticles for cancer drug delivery. Nanoscale Advances. 2021, 3(8): 2157-2179.
Bernard, M.C.; et al. The impact of nucleoside base modification in mRNA vaccine is influenced by the chemistry of its lipid nanoparticle delivery system. Molecular Therapy-Nucleic Acids. 2023, 32: 794-806.
Jordheim, L.P.; et al. Advances in the development of nucleoside and nucleotide analogues for cancer and viral diseases. Nature reviews Drug discovery. 2013, 12(6): 447-464.

This service is for Research Use Only, not intended for any clinical use.