OPME-based Nucleotide Monophosphate (NMP)-sugar Synthesis Service

CD BioGlyco has developed glycosyltransferase-catalyzed enzymatic and chemoenzymatic syntheses. We provide robust solutions for the synthesis of Oligosaccharides, polysaccharides, glycoconjugates, and derivatives. We utilize OPME systems to seamlessly integrate enzymes from sugar nucleotide biosynthesis with glycosyltransferases, allowing the efficient synthesis of diverse glycans. Characterized by substrate promiscuity, this approach facilitates the generation of complex carbohydrates, both natural and modified. CD BioGlyco's sequential application of OPME strategies defines glycan structures, ensuring versatility in addressing diverse glycomes with precision and simplicity.

• NMP-sugar synthesis service at CD BioGlyco

The OPME-based NMP-sugar synthesis is a streamlined enzymatic process developed by CD BioGlyco. In this approach, various enzymes are combined in a single reaction vessel to catalyze the sequential synthesis of NMP-sugars from simple precursors. This one-pot strategy minimizes the need for intermediate purifications, enhancing the overall yield and efficiency of NMP-sugar synthesis for various applications in glycan-related products and services.

Fig.1 The process of OPME-based NMP-sugar synthesis. (CD BioGlyco)

The OPME-based NMP-sugar synthesis service at CD BioGlyco involves the following steps. Our synthesis service provides a streamlined and comprehensive solution for tailored and precise synthesis needs in glycan-related applications.

• Enzyme selection: Identification and selection of enzymes involved in sugar nucleotide biosynthesis, ensuring compatibility and efficiency in the synthesis process.
• Reaction setup: Combining various enzymes in a single reaction vessel, creating an environment conducive to multiple enzymatic steps occurring simultaneously.
• Substrate introduction: Introducing precursor substrates, including modified monosaccharides, into the reaction mixture to initiate the synthesis process.
• Catalysis: Enzymatic catalysis of sugar nucleotide biosynthesis, facilitated by the selected enzymes, leading to the formation of NMP-sugars.
• Sequential operations: The option for sequential operations, allowing for the synthesis of complex carbohydrates with defined structures.
• Product isolation: Efficient isolation of the synthesized NMP-sugars, minimizing the need for intermediate purifications.
• Quality control: Rigorous quality control measures to ensure the purity and structural accuracy of the synthesized NMP-sugars.

After synthesis and purification, we also provice Modification Service for clients according to their needs. Moreover, we provide other high-quality Nucleotide-based Production Service for our clients to choose.

Publication Data

Applications

• NMP-sugars serve as crucial substrates in glycosylation reactions, playing a pivotal role in the biosynthesis of oligosaccharides, polysaccharides, and glycoconjugates.
• NMP-sugars are utilized in studies focused on understanding glycan structures, functions, and their implications in health and disease. They contribute to advancements in glycobiology and biomarker discovery.
• NMP-sugars are involved in the synthesis of glycan-based therapeutics and drug candidates. They play a role in the design and development of drugs targeting glycan-related processes.
• Glycans are important components of many pathogens, and NMP-sugar synthesis is crucial in the development of glycan-based vaccines. This includes vaccines for bacterial and viral infections.

Advantages

• This one-pot approach reduces the need for intermediate purifications, streamlining the overall process and increasing efficiency.
• Enzyme-catalyzed reactions in OPME exhibit high stereo- and region-selectivity. This specificity ensures the precise formation of desired glycan structures, reducing the likelihood of unwanted by-products.
• OPME can be a cost-effective method for large-scale glycan synthesis, making it attractive for industrial applications.

CD BioGlyco is expertise in NMP-sugar synthesis and offers versatile solutions for researchers and industries involved in these diverse applications, providing tailored Nucleoside & Nucleotide products for specific needs. Please feel free to contact us for more details.