Caragana arborescens Lectin (CAA) Production Service at CD BioGlyco

CD BioGlyco is committed to providing efficient Lectin Production Services. Plant Lectins are readily available soluble proteins that are easily produced. Due to its inherent specificity, it has a variety of biological functions. Researchers purified and characterized two lectins from C. arborescens. These two lectins are believed to play a role in nitrogen fixation. We provide a one-stop CAA production service to facilitate clients' research on CAA.

Fig.1 Process of CAA production. (CD BioGlyco)

• CAA production service

We mainly extract lectins from the seeds of C. arborescens. Using C. arborescens seeds as the material, the optimized extraction method greatly shortens the production cycle.

Common purification methods for lectins include Ion Exchange Chromatography, Hydrophobic Chromatography, Affinity Chromatography, etc. We use affinity chromatography to purify the crude extract. Lectin purification may suffer from insufficient purification and low production efficiency. The establishment of purification methods that obtain high purity and high yield of lectins is one of the prerequisites for smooth lectin production. Based on the reported steps, we optimize the purification method.

According to client requirements, we perform a series of tests on the lectin after purification. The molecular weight is detected by sodium dodecyl-sulfate polyacrylamide gel electrophoresis. Its amino acid composition is determined by an amino acid analysis system. The N-terminal amino acid sequence is determined by high-performance liquid chromatography. We also perform a lectin sugar-binding specificity assay.

• CAA products

After optimized extraction and purification methods, we obtain high-quality CAA and conjugate products. Our products include CAA, colloidal gold conjugate, biotin conjugate, ferritin conjugate, FITC conjugate, etc.

Applications of CAA Production

• Plant lectins have excellent growth inhibitory effects on a wide range of fungi and bacteria. CAA produced by our optimized method is used for research on bacterial inhibition.
• CAA produced by our optimized method is used for the development of efficient biotechnologies, such as immunofluorescence and protein blotting.
• The CAA produced by our optimized method is used in cancer research.

Advantages of Us

• Affinity chromatography is currently the most ideal way to purify proteins, with the advantages of rapidity, efficiency, and simplicity. We use affinity chromatography to purify lectins.
• We use an optimized production method to extract lectins from C. arborescens seeds. The extraction method is simple and efficient, which reduces the production time.
• The various CAA products obtained through our optimized production method meet the different research directions and routine experimental needs of our clients.

CD BioGlyco produces high-quality lectins. We aim to provide our clients with efficient lectin production services. Please feel free to contact us for detailed information about CAA product parameters, production steps, etc. We will provide you with one-to-one answers.