Cell Surface Hydroxy Fatty Acid (HFA) GPI Anchor Glycoengineering Service at CD BioGlyco

CD BioGlyco is committed to facilitating clients in Glycoprotein research. GPI is a glycolipid widely found in eukaryotes. It contains fatty acid chains and plays an essential role in life activities. We provide cell surface HFA GPI anchor glycoengineering service.

Fig.1 Process of a one-stop HFA glycoengineering. (CD BioGlyco)

• Targeted modification of cell surface HFA GPI anchor

We mainly perform targeted modification of cell surface HFA GPI anchor through gene editing. The structure of HFA in GPI-AP is altered by regulating the synthesis of key enzyme genes, including Gene Knockout and Gene Overexpression. It is found that the structural alteration of HFA in GPI anchor protein (GPI-AP) affects the development of plant seed coat and so on. The role of this GPI-AP in growth and development can finally be detected by this method.

• HFA GPI anchor structure analysis

The analysis of hydroxy fatty acids is based on the identification of the 2-hydroxy group and the position of the double bond in the fatty chain. The 2-hydroxyl group in the carbon chain of fatty acids is close to the carbonyl end of the fatty chain and is easier to identify in the mass spectra of their methyl ester derivatives. The main derivatization methods currently used for fatty acid double bond analysis are 2-amino-2-methylpropanol derivatization, o-aminophenol derivatization, and dimethyl disulfide derivatization. We derivatize HFA in GPI anchors by acid methyl esterification derivatization, hydroxy acetylation protection, dimethyl disulfide derivatization, etc. These methods have certain drawbacks, such as requiring higher reaction temperatures and not being easy to identify. Therefore, we optimized the derivatization method of HFA and achieved the desired derivatization results. We use gas chromatography-mass spectrometry to analyze its 2-hydroxy fatty acid composition.

Applications

• GPI-AP has an important role in plant growth and development. The effects of HFA changes on plant seed growth, flower development, and other activities can be detected by gene regulation.
• The hydroxyl group in 2-hydroxy fatty acids helps to stabilize the cell membrane structure and enhance the interaction between lipids and membrane proteins. HFA analysis is used to study its role in life activities.
• Abnormalities in HFA may cause abnormalities in vital activities in the body. HFA analysis has been used to study its correlation with the development of disease. HFA analysis has been used to study its correlation with the development of disease.

Highlights

• GPI-AP alterations can have an impact on the growth and development of organisms. Gene editing technology helps to study its physiological function and regulate the growth and development of organisms.
• The reported HFA derivatization method has some drawbacks such as requiring higher reaction temperature, not being easy to identify, etc. We optimized the derivatization method to improve the accuracy of structural analysis.
• We used efficient mass spectrometry for the analysis, which greatly shortened the detection period. The experimental results are accurate with low error.

CD BioGlyco is experienced in Glycoengineering. We aim to facilitate the research of GPI Anchor. Welcome to contact us to tell us your needs. We will complete all cell surface HFA GPI anchor glycoengineering experiments. We sincerely look forward to working with you to help your research.