CD BioGlyco is experienced in Lectin Production, such as the production of Bauhinia purpurea Lectin, Colchicum autumnale Lectin, etc. Lectin is a blood-clotting protein. They are found in a variety of plants. Studies find that Plant Lectins have a variety of biological activities, including antifungal, anticancer, etc. They are relatively easy to produce. Currently, there is much research on CAL, including lectin gene cloning, bioactivity detection, transgenic plants stably expressing lectins, and so on. We provide a one-stop CAL production service to meet the needs of clients in related research.
The seeds of leguminous plants contain more lectins. We mainly use C. arietinum seeds as materials to extract lectins. The crude extract of lectin is obtained by Tris-HCl extraction. Afterward, purification is carried out by precipitation and dialysis. Ion Exchange Chromatography has a very high resolution, high recovery, and a wide range of applications. We use ion exchange chromatography for further purification of lectins.
The molecular weight of C.arietinum lectins varies from species to species. The physicochemical properties of the extracted lectins will be tested according to client requirements. We have advanced types of equipment such as amino acid auto analyzer, Fourier-transform infrared spectroscopy, etc. Amino acid composition, sugar-binding specificity analysis, secondary structure detection, molecular weight detection, etc are all accomplished.
Fig.1 Process of CAL production. (CD BioGlyco)
We offer a wide range of CAL products based on optimized production methods. In addition to CAL, we also have a variety of lectin conjugate products, such as alkaline phosphatase conjugate, peroxidase conjugate, ferritin conjugate, agarose conjugate, etc.
| Cat. No. | Product Name | Cat. No. | Product Name |
|---|---|---|---|
| XLC-17-03Q | CAL, Alkaline Phosphatase Conjugate | XLC-17-12Q | CAL, FITC Conjugate |
| XLC-17-04Q | CAL, Biotin Conjugate | XLC-17-14Q | CAL, Peroxidase Conjugate |
| XLC-17-05Q-10 | CAL, Colloidal Gold Conjugate, 10 nm | XLC-17-01Q | CAL, Unconjugate |
| XLC-17-05Q-15 | CAL, Colloidal Gold Conjugate, 15 nm | XLC-17-02Q | CAL, Agarose Conjugate |
| XLC-17-05Q-20 | CAL, Colloidal Gold Conjugate, 20 nm | XLC-17-15Q | CAL, Rhodamine Conjugate |
| XLC-17-05Q-30 | CAL, Colloidal Gold Conjugate, 30 nm | XLC-17-16Q | CAL, Cy3 Conjugate |
| XLC-17-05Q-40 | CAL, Colloidal Gold Conjugate, 40 nm | XLC-17-17Q | CAL, Cy5 Conjugate |
| XLC-17-05Q-5 | CAL, Colloidal Gold Conjugate, 5 nm | XLC-17-18Q | CAL, Texas Red Conjugate |
| XLC-17-11Q | CAL, Ferritin Conjugate | X23-02-ZQ332 | CAL, Alexa Fluor 647 Conjugate |
Technology: Ion Exchange Chromatography
Journal: Physiology and Molecular Biology of Plants
IF: 2.005
Published: 2018
Results: In the present study, C. arietinum seed powder was used as raw material for the extraction of lectins. Tris-HCl extraction was followed by dialysis. This was followed by further purification by sequential ion exchange chromatography including diethylaminoethyl (DEAE)-cellulose and Sulphopropyl (SP)-Sephadex matrix. The lectin was finally successfully extracted. The molecular weight of the lectin subunit was determined to be 35 kDa. The molecular weight of the lectin in the seeds of different varieties of C. arietinum may vary.
Tab.1 Table depicting yield of lectin during different purification steps. (Gautam, et al., 2018)
| Purification steps | Total protein (mg/100 g seeds) |
Hemagglutination unit (HAU) |
Specific activity (HAU/mg protein) |
Purification (fold) |
Yield (%) |
|---|---|---|---|---|---|
| Crude extract | 1600 | 8192 | 512.10 | 1 | 100 |
| 20–80% ammonium sulfate fraction | 750 | 4096 | 546.13 | 1.06 | 50 |
| DEAE-cellulose fraction | 455 | 4096 | 900.21 | 1.75 | 25 |
| SP-Sephadex fraction | 95 | 2048 | 2155.78 | 4.20 | 25 |
CD BioGlyco strictly controls every aspect of lectin production. We aim to provide clients with efficient plant lectin production services. To provide more kinds of lectin products to our clients, we are constantly improving our production capacity. Please feel free to contact us to inquire about the price, parameter information, and production method of our lectin products. We are ready to provide you with lectin production services at any time.
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