The CPS Structure of Two Types of E. coli

The capsular polysaccharide (CPS), which gives enhanced resistance to host defenses such as complement-mediated death and phagocytosis, is a crucial virulence component during invasive infections. Furthermore, the similarity of certain CPS to host molecules enables bacteria to employ a molecular mimicry technique, which further supports survival. Sometimes the capsule is made of polymers that resemble glycosaminoglycans (GAGs). GAGs are long unbranched polysaccharides found in vertebrates that are typically present on the surface of cells or in the extracellular matrix, where they play an important role in cell growth, adhesion, differentiation, and proliferation. GAGs, including chondroitin sulfate (CS), heparin, and hyaluronic acid, are economically lucrative biopolymers with a variety of uses in the medical, veterinary, pharmaceutical, and cosmetic industries. Chondroitin-like polysaccharides were found to be suitable substrates for GlcA- and GalNAc-transferase activities in E. coli K4, indicating their potential involvement in capsule polysaccharide synthesis.

Fig.1 Structures of the bacterial CPS. (A) E. coli K5. (B) E. coli K4. (C) enzymatic degradation of the [³H]GalNAc-labeled product. (D) enzymatic degradation of the [¹⁴C]GlcA-labeled product. (Lidholt & Fjelstad, 1997)

E. coli CPS Production Service at CD BioGlyco

CPS production has traditionally relied on isolating it from animal tissues, which can be challenging due to the risks of viral and prion contamination and the associated high costs. In 1996, the first study on cultivating E. coli K4 and exploring the structure of its cell-bound and cell-free K4 polysaccharide was published. However, there is currently limited research on the physiological aspects of E. coli that connect dietary requirements and growth conditions to CPS production.

At CD BioGlyco, we understand the importance of CPS in various applications. That's why we offer a specialized E. coli CPS production service for our clients. With our state-of-the-art laboratory facilities and expertise, we can provide tailored CPS production solutions, including specific strains like K4 strains. By leveraging our resources, we can help meet your CPS production needs efficiently and effectively.

Fig.2 The standard flowchart of E. coli CPS production. (CD BioGlyco)

Advantages of Us

• Our skilled scientists and experienced laboratory staff are able to perform our analytical tests and subsequent data processing to a high standard, offering our clients reliable laboratory outcomes.
• Our analytical platform provides a wide range of high-quality, excellent, and suitable custom QC for our glycochemical products.
• We provide different E. coli strain CPS according to our client's needs.

CD BioGlyco has a service team who consistently receives positive feedback from clients, reflecting our commitment to providing exceptional service and our dedication to exceeding customer expectations. If you have any questions or require detailed information, please feel free to contact us.

Reference:

1. Lidholt, K.; Fjelstad, M. Biosynthesis of the Escherichia coli K4 capsule polysaccharide. Journal of Biological Chemistry. 1997, 272(5): 2682–2687.