Lymphoma and Glycosylation

Lymphoma is the sixth most common cancer. Increased metabolic activity of lymphoma tumor cells associated with high expression of glycolysis-related proteins, has already been described and indicates a pivotal role for glucose and glycogen metabolism in the malignant progression of the disease. Studies have shown that glycosylation is a key regulator for the distant metastasis of mouse lymphoma cells in animal models through using mouse lymphoma cell lines. In addition, the concentration of sialic acid has been reported to be elevated in the serum of patients with malignant lymphoma, suggesting that sialic acid may be associated with tumor cell aggressiveness in lymphoma.

Fig.1 Increased expression and activation of the molecules that promote tumor cell survival and modulate glucose/glycogen metabolism. (Jalali & Ansell, 2020)

Glycogene Discovery Service in Lymphoma

To support Cancer Glycogene research, CD BioGlyco offers glycogene discovery services in lymphoma and other cancers. The services we provide include but are not limited to:

• Discovery methods
  • Glycoprotein Structure Analysis
    Mass spectrometry (MS), matrix-assisted laser desorption/ionization (MALDI-MS), liquid chromatography (LC), etc.
  • Identification and quantification of glycopeptides
    Analyzed by LC-MS/MS
  • Glycogene Expression Profiling Analysis
    The quantitative real-time polymerase chain reaction (qRT-PCR) and RNA sequencing (RNA-Seq)
  • Glycan Analysis
    • Oligosaccharide separation: capillary electrophoresis (CE)
    • Glycan structures: MS, high-performance liquid chromatography (HPLC)
    • Characterization and Quantitation of Glycan: liquid chromatography-electrospray ionization MS (LC/ESI-MS), matrix-assisted laser desorption/ionization-time of flight MS (MALDI-TOF-MS)
    • Capture of specific glycan of the glycoproteins: Lectin-based technologies: Lectin Affinity Chromatography and lectin microarray
• Summary of glycogenes involved in lymphoma
  • Glucose analogue ¹⁸F-fluoro-2-deoxy-D-glucose (¹⁸F-FDG)
  • Glucose transporters (GLUTs)
  • Hexokinases (HK)
  • Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
  • Glycogen synthase kinase 3 (GSK3)

Fig.2 Example of the discovery process in lymphoma. (CD BioGlyco)

Applications

• Characterizing the glycosylation of therapeutic glycoproteins is an important way to monitor the batch-to-batch consistency of the manufacturing process.
• Glycopeptide analysis of proteins is used to develop 3D models of intact proteins, including glycoforms that determine the properties of glycoproteins.
• Lectin-based technologies are applied to capture the specific glycan of the glycoproteins, enhancing the understanding of aberrant glycosylation and carcinogenesis, and accelerating biomarker recognition and quantitative detection.

Advantages

• High-throughput, high-speed, and high-capability. 
• Professional advice from our analysis scientists.
• Customized services according to your requirements.
• Combination of multiple advanced analysis platforms.

Over the years, with constant collaboration and innovation, CD BioGlyco has enriched the Glycogenomics Platform to cover our client's essential needs. We continue to conduct research and expand our service area to help our clients achieve their goals. In addition, our expert team has extensive experience in Glycogene Editing and Glycogene Delivery. Welcome to contact us for custom service. We look forward to your happy cooperation.