Advantages of ¹H,¹H-COSY Test

It is an essential idea of ¹H,¹H correlated spectroscopy (or ¹H,¹H-COSY) to connect protons that are scalarly (or through-bonds) coupled and have a coupling constant value (J) different than zero. With the exception of some precise geometric conditions, which is seldom the case in carbohydrates, these protons are normally separated by two (2J) or three (3J) chemical bonds, whereas protons separated by four linkages (or more) are often not connected. Like other homonuclear spectra, the COSY spectrum is symmetrical around the diagonal, therefore its densities may be separated into those that form the diagonal and those that are off the diagonal.

Fig.1 Methyl-β-D-glucopyranoside unit (labeled A) with the indication of the information gathered by homonuclear ¹H,¹H-COSY experiments. (Speciale, et al., 2022)

¹H,¹H-COSY GSL Structure Analysis Service at CD BioGlyco

A useful method for identifying signals that are generated by adjacent protons often up to four bonds is ¹H,¹H-COSY. Protons show correlations when spin-spin interaction is present. However, where there is no connectivity, no correlation is expected. The most popular type is DQF-COSY, which has gradient-enhanced double-quantum coherence.

Fig.2 NMR in carbohydrate structural analysis. (Speciale, et al., 2022)

CD BioGlyco establishes a powerful GSL structure analysis platform with good equipment and a well-organized research team. Because GSL structure analysis is difficult to clarify by one analysis method, normally is analyzed by multiple methods. On our website, clients can find NMR-based analysis services not only ¹H,¹H-COSY analysis service but also ¹H NMR Analysis Service, ¹³C NMR Analysis Service, ¹H,¹H-TOCSY Analysis Service, ¹H,¹H-ROESY Analysis Service, and so on. Here is a standard NMR-based mapping analysis solution for reference in Fig. 3. Our skilled scientists and professional laboratory staff are able to carry out our analytical tests and subsequent data analysis in fantastic quality, providing our clients with reliable laboratory reports.

Fig.3 Mapping analysis solutions. (CD BioGlyco)

Advantages of Us

• We offer suitable custom GSL structural analysis services such as NMR-based and MS-based for clients according to their requirements and budgets.
• We provide custom GSL analysis solutions for our clients based on our test conditions and clients’ needs.
• We employ outstanding lab operators to handle these analysis experiments and aim to provide excellent services for our clients.

CD BioGlyco has extensive research experience in glycan study, our research team provides professional and custom NMR-based analysis services for our clients. Please feel free to contact us for more information if you are interested in our analysis services.

Reference

1. Speciale, I.; et al. Liquid-state NMR spectroscopy for complex carbohydrate structural analysis: A hitchhiker’s guide. Carbohydrate Polymers. 2022, 277: 118885.