¹H,¹H-TOCSY Analysis Methods

The preferred methods for analyzing stable isotope labeling patterns in cell metabolites are NMR and MS. The biosynthetic network may be quantitatively described by the measurement of isotope enrichment in metabolites, which also permits flow analysis in reaction to outside disruptions. Total correlation spectroscopy (TOCSY), like COSY, is a homonuclear 2D analysis. Contrary to COSY, the identification of related spins is not limited to immediate neighbors. By altering the isotropic mixing scenario during spin-locking, the TOCSY experiment creates cross-peaks between all hydrogen atoms that are part of a continuous chain of linked spins.

Fig.1 2-D ¹H TOCSY spectra of components of UDP-N-acetyl-D-glucosamine (UDP-GlcNAc). (a) Ribose ring region of the TOCSY spectrum. (b) The uracil ring in UXP shows the scrambled pattern in the C5-C6 positions of U. (Moseley, et al., 2011)

¹H,¹H-TOCSY Analysis Service at CD BioGlyco

The more well-known COSY experiment is usefully supplemented by the 2D TOCSY experiment. TOCSY can offer correlations between all of the protons that make up a spin system, whereas COSY often develops correlations between protons spanning two to three chemical bonds. Unlike COSY, which only establishes correlations between geminal or vicinal protons, TOCSY establishes correlations between all protons in a particular spin system. As long as there exist couplings between each intervening proton, correlations between far-off protons can be observed. This is very helpful for locating protons on amino acids or sugar rings: The connection between every proton on a given sugar ring and every other proton on the same ring will always be the same, but not for protons on different rings.

CD BioGlyco provides not only COSY analysis services but also high-quality 2D TOCSY analysis services. Since the structural analysis of different sugar molecules requires the use of different analytical methods, our powerful polysaccharide analysis platform offers a variety of NMR-based Analytical Custom Services and MS-based Analytical Custom Services to provide our clients with outstanding structural characterization solutions.

Fig.2 The development of COSY to 2D TOCSY. (CD BioGlyco)

Advantages of Us

• Our service team is available to respond to inquiries from clients around the world promptly.
• Our analytical platform provides a wide range of high-quality, excellent, and suitable custom analysis services for glycochemical analysis.
• Our talented scientists and well-experienced laboratory team perform our analytical experiments and subsequent data analysis in excellent quality, providing our clients with reliable laboratory reports.

CD BioGlyco has extensive research experience in glycan study, our research and service team provides professional and custom NMR-based analysis services and MS-based analysis services for our clients. if you have any questions or require details information, please feel free to contact us.

Reference

1. Moseley, H.N.; et al. A novel deconvolution method for modeling UDP-N-acetyl-D-glucosamine biosynthetic pathways based on 13C mass isotopologue profiles under non-steady-state conditions. BMC Biology. 2011, 9(1): 37.