Biological Significance and Applications of N-Glycan

N-glycans are glycans attached to proteins. N-glycans are found in a variety of organisms, including animals, plants, and microorganisms, and are significant for protein folding, stability, function, and cellular recognition. They are also involved in various processes including inflammation, immune response, and cancer metastasis. Dysfunctional N-glycosylation has been associated with a range of diseases which include cancer, congenital glycosylation disorders, and neurodegenerative diseases. N-glycans have a variety of physical and chemical characteristics, such as water retention, viscosity, gelling properties, and stability, and are therefore often used as materials in medicine, cosmetics, food, and industry. For example, some N-glycans have biological activities such as antioxidant, immunomodulatory, anti-inflammatory, and antibacterial, which can be used in the preparation of nutraceuticals and drugs.

Fig.1 Workflow for high-throughput N-glycan screening. (Kinoshita, et al., 2021)

High-Throughput Screening (HTS) of N-Glycan Service at CD BioGlyco

N-glycans play a variety of critical roles in biology, providing the basic functional and structural support for living organisms. Therefore, the research on N-glycans is important for understanding and diagnosing diseases as well as developing drugs. CD BioGlyco focuses on diverse research in the field of glycobiology, including glycan analysis. We strive to provide efficient and cost-effective high-throughput N-glycan screening services for our global clients. Our N-glycan screening services include but are not limited to those listed below:

• HTS of N-glycan by Microchip-Based Assay
  We analyze hundreds to thousands of N-glycan samples and detect N-glycan structures down to 5 fmol, which is ideal for biomedical research projects where high-throughput screening is being performed.
• HTS of N-glycan by Miniature Pig Kidney N-Glycan -Immobilized Beads
  This technology uses low amounts of reagents and can be combined with other techniques for in-depth analysis of specific N-glycosylations.
• HTS of N-glycan by 19F-Labeled Probes
  We provide high sensitivity, low background, and high resolution biological nuclear magnetic resonance (NMR) spectra, enabling the differences therein to be used for rapid separation and identification of different N-glycosylation types.

Fig.2 Technologies for HTS of N-Glycan. (CD BioGlyco)

Applications

• Detection and identification of glycosyl markers associated with specific diseases
• Uncovering the function and mechanism of action of sugar groups
• Bio-detection and signaling in biosensors

Advantages of Us

• High sensitivity, low background, and high resolution
• High specificity and accuracy
• Detailed data processing and analysis of results

CD BioGlyco provides customized high-throughput N-glycan screening services based on a variety of methods. Our professional, timely, and efficient after-sales service has resulted in very high client satisfaction. If you are interested in our services, please contact us for more details.