HSA consists of a single chain of 585 amino acids. Its secondary structure is highly flexible, with 67% α-helices and 17 disulfide bridges with 6 turns, acting as cross-linkers for the three homology domains. It acts as a free radical scavenger under inflammatory conditions and is involved in processes such as blood clotting and wound healing. Furthermore, it has antioxidant properties and heparin-like activity by enhancing antithrombin IIl activity. The albumin molecule has many charged amino acid residues. Therefore, sodium ions and other cations are strongly attracted to albumin.
Fig.1 Structure of HSA. (Spada, et al., 2021)
CD BioGlyco provides high-quality HSA and polysaccharide conjugation services. Our conjugation services include but are not limited to providing polysaccharide-HSA conjugation technology and characterization. Generally, the workflow of our polysaccharide-HSA conjugation service is as follows.
According to the needs of the experiment, suitable polysaccharides and HSA are obtained. HSA is filtered through deionized water at room temperature, sterile filtered, and freeze-dried before experiments.
Molecular weights of HSA and polysaccharides are determined by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The polysaccharide is activated with cyanogen bromide and derivatized with adipic dihydrazide, and conjugated to HSA by carbodiimide condensation. The reaction mixture is allowed to contain equal amounts of HSA and adipic hydrazide derivatives of polysaccharides. 1-ethyl-3-dimethylaminopropylcarbodiimide is added and the reaction mixture is kept slightly acidic. The mixture is dialyzed overnight and loaded onto the column. The composition of the polysaccharide-HSA conjugate is determined by chemical analysis. Fractions appearing in the column void volume are pooled and used to vaccinate mice.
Female mice are subcutaneously injected into groups. Bleeding is performed after the number of injections is reached. Mice injected with saline are used as a control group. Then ELISA assay is performed.
Fig.2 Polysaccharide-HSA conjugation service. (CD BioGlyco)
CD BioGlyco provides an integrated solution to develop carbohydrate-based vaccines according to each client's scientific needs. We have various advanced technology platforms and high-quality services to meet your requirements. If you are interested in our services, please contact us for more details without any hesitation.
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