Maackia amurensis Lectin (MAL) Production Service at CD BioGlyco

CD BioGlyco is a lectin research, Production, and sales company. We are committed to providing outstanding Plant Lectin Production service and support to our clients. MAL research is extensive. Our rich production experience provides a one-stop MAL production service and a wide range of lectin products.

Fig.1 Process of MAL production. (CD BioGlyco)

• MAL production service

Two lectins, MAL-I and MAL-II, have been extracted from the seeds of M. amurensis. They have agglutinating activity on different blood cells and binding specificity to Neu5Acα2-3. Researchers use them as analytical tools for α2-3-linked sialic acids biological targets. The production of lectins is fundamental to the study of their physiological functions and applications. We optimize them according to the reported MAL extraction method.

Lectins are easily affected by the extraction time, material-liquid ratio, concentration, and pH of the extraction Solution. We mainly use M. amurensis seeds and bark as experimental materials to optimize the conditions of concentration of extraction solution, material-liquid ratio, extraction time, and pH of lectin. After extraction by Tris-HCI buffer, the extracted lectins are treated by graded precipitation with ammonium sulfate. Afterward, it is further purified using a combination of Gel Chromatography and Ion Exchange Chromatography. Finally, the purified lectin product is obtained by freeze-drying.

We measure the purity and characterize the obtained lectins. In addition to this, agglutination activity, sugar-binding specificity, amino acid composition, etc., are also measured.

• MAL products

After testing, our optimized extraction and isolation purification process is simple and the resulting product is of high purity.

Due to the specific carbohydrate binding of lectin, it is used to study cell surface glycoproteins. Couplings of lectin with fluorescent substances are used for lectin staining. In addition to MAL, we offer a wide range of lectin-conjugated products.

Applications of MAL Production

• The MAL we produced is a glycoanalysis tool to detect α2-3-linked sialic acids biological targets.
• Assays using lectins as glycosylation recognition sites have become new indicators for early tumor diagnosis. The MAL we produce is used to study tumor detection methods.
• Fluorescent lectins are used to localize and detect glycoconjugates. Our MAL conjugates are effective assay reagents that are used for microscopy or flow cytometry.

Advantages of Us

• We have a diverse range of MAL and its conjugates to meet the different research needs of our clients for the detection of glycoconjugates, visualization localization, etc.
• Our optimized extraction separation and purification processes are simple. The resulting products are of high purity.
• We have an experienced and highly qualified team of lectin producers ready to provide our clients with a wide range of production services.

CD BioGlyco has a comprehensive quality monitoring system for lectin production. We are determined to provide first-class products for our clients and support their research with efficient and high-quality products and services. In addition to MAL, we also provide production for Lotus tetragonolobus Lectin, Lycopersicon esculentum Lectin, etc. Please feel free to contact us for detailed information about MAL products such as price and production process. Our technical and sales staff will contact you as soon as possible.

Reference:

1. Geisler, C.; Jarvis, D.L. Letter to the Glyco-Forum: Effective glycoanalysis with Maackia amurensis lectins requires a clear understanding of their binding specificities. Glycobiology. 2011, 21(8): 988-993.