Comprised of metal elements, either in isolated or composite form, and their oxides, magnetic glyconanoparticles, particularly superparamagnetic magnetite, have emerged as a staple due to their superior biocompatibility and minimal toxicity. These iron oxide-based magnetic glyconanoparticles have increasingly captivated the scientific community, fostering advancements in understanding and application across various domains. Smaller-sized iron oxide magnetic glyconanoparticles are particularly favored in biological and biomedical applications. CD BioGlyco uses the advanced GlycoNano™ Platform, providing customized development and production of magnetic glyconanoparticles that integrate the controllable properties of magnetic materials with the biocompatibility and specificity of carbohydrate molecules. The surface chemistry of these magnetic glyconanoparticles is meticulously modulated to refine their physicochemical characteristics, enabling their deployment in diverse fields.
Among these, we perform a series of meticulously designed steps to create high-quality, mannose-coated maghemite nanoparticles.
We synthesize iron oxide nanoparticles by co-precipitating iron chloride precursors in deoxygenated water, followed by the addition of ammonium hydroxide. Wash the resulting black precipitate to adjust the pH to neutral.
Allow the solution to sediment under a magnetic field, discarding the supernatant. The black sediment is mixed with nitric acid and ferric nitrate, followed by dialysis against nitric acid.
We mix the treated nanoparticles with mannan to coat the nanoparticles, adjusting the pH to neutral using ammonium hydroxide. This coating step is crucial for enhancing the nanoparticles' performance in various applications.
After synthesis, characterization of the mannan-coated magnetic nanoparticle is conducted to determine key properties. We thoroughly assess the glyconanoparticles through two key components: the Glyconanoparticle Physicochemical Property Characterization Service, which evaluates the nanoparticles' size, charge, stability, and surface properties, and the Glyconanoparticle Molecule Interaction Analysis Service, which investigates the interactions between the glyconanoparticles and various biomolecules, ensuring optimal performance and compatibility in biomedical applications.
Technology: Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM)
Journal: 10.3390/catal9010007
IF: 10.6
Published: 2019
Results: In this study, the researchers optimized D-mannose-coated magnetic Fe3O4@OA@DP nanoparticles for immobilizing Escherichia coli cells that express recombinant glycerol dehydrogenase. TEM showed that the nanoparticles increased in size after mannose functionalization and effectively immobilized the E. coli cells. The optimal conditions for immobilization included a specific pH, cell concentration, and temperature, which maximized both yield and activity recovery. A certain buffer concentration was also found to be ideal for activity recovery. The immobilized cells performed better at higher pH and temperatures, retaining a high percentage of their initial enzyme activity after several hours of incubation, compared to a significant drop in free cells. Additionally, the immobilized cells maintained a substantial portion of their activity after multiple cycles, far outperforming free cells that retained only a minimal fraction of activity. The authors used the immobilized cells to convert glycerol to 1,3-dihydroxyacetone (DHA), achieving a significant improvement in production rate. This method eliminates the need for enzyme purification or expensive cofactors, reducing costs and making the immobilized cells highly promising for industrial applications, especially in DHA production.
CD BioGlyco offers high-quality and comprehensive Glyconanoparticle development Service via advanced techniques, ensuring superior biocompatibility and minimal toxicity. The modulated surface chemistry of our magnetic glyconanoparticles enables their deployment in a wide range of fields, from diagnostics research to targeted drug delivery research. If you want to obtain high-quality, mannose-coated magnetic nanoparticles, tailored to meet the specific needs of various scientific and industrial applications, contact us!
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