Non-natural NeuAc Analog-based Cell Surface Metabolic Oligosaccharide Engineering Service

Non-natural NeuAc Analog-based Cell Surface Metabolic Oligosaccharide Engineering Service at CD BioGlyco

CD BioGlyco has a specialized team of Salivary Acid researchers. Sialylated glycans are recognized by exogenous and endogenous receptors. They play important roles in physiology and pathology, respectively. An important tool for studying the function and localization of sialylated glycans in living cells, etc., is Metabolic Oligosaccharide Engineering (MOE). MOE is a rapidly developing technology in chemical biology. It allows the biosynthesis of new chemically functional moieties into carbohydrates of living cells and animals. We offer cell surface MOE services based on non-natural NeuAc analog.

We incorporate new chemical functional groups into NeuAc, including azides, ketones, alkynes, thiols, etc. Analogs greatly expand the potential of MOE to control biological processes due to the incorporation of chemical functional groups not normally found in carbohydrates. Through MOE, NeuAc analogs carrying unnatural modifications are provided to cells and incorporated into cell surface glycans. This compound can later be visualized by bioorthogonal reactions with fluorescent molecules, etc.

Fig.1 Process for cell surface MOE based on non-natural NeuAc analogs. (CD BioGlyco)

Publication

Paper Title: Switching azide and alkyne tags on bioorthogonal reporters in metabolic labeling of sialylatedglycoconjugates: a comparative study

Technology: MOE, Copper-catalyzed Azide-alkyne Cycloaddition (CuAAC)

Journal: Scientific Reports

IF: 4.997

Published: 2022

Results: The sialic acid family includes several species such as N-acetyl neuraminic acid (Neu5Ac, Sia). In this study, the metabolic incorporation of ManNAl, ManNAz, SiaNAl, SiaNAz, and per-O-acetylated ManNAz in several cell lines was compared using CuAAC. The results revealed that there were significant differences in the labeling efficiencies of these five chemical reporters. Conversion of alkynes and azide bioorthogonal labeling on analogs greatly affects their metabolic incorporation in human colon cell lines.

Fig.2 Comparison of the efficiency of metabolic incorporation of the analogs by western blot. (Scache, et al., 2022)

Applications

• MOE using NeuAc is useful for studying defects in the sialic acid biosynthesis and recycling pathway.
• Cell surface MOE based on non-natural NeuAc analog plays an important role in the study of metastatic mechanisms in cancer cells.
• Cell surface MOE based on non-natural NeuAc analog plays an important role in the study of cell signaling pathways.

Advantages

• Our non-natural NeuAc analog-based cell surface MOE service helps clients manipulate glycans on the cell surface of various cells and living organisms.
• Our cell surface MOE service based on a non-natural NeuAc analog can help clients study the localization, expression, and transport of sialic acid glycans.
• Our various modifications of NeuAc greatly expand the range of applications of cell surface MOE based on non-natural NeuAc analogs.

CD BioGlyco is skilled in utilizing Cell Surface MOE for various studies. In addition to non-natural NeuAc analog, we also provide services such as Non-natural Fuc Analog-based MOE service, Non-natural GalNAc Analog-based MOE service, etc. Welcome to contact us for more information about the non-natural NeuAc analog, MOE detailed program. We are ready to serve you at any time.