Molecular beacons are important bioanalytical probes, most often constructed from single-stranded oligonucleotides that are labeled with a fluorophore and a bursting agent at the opposite end. It depends on bursting fluorescence by transferring energy from one fluorophore to another fluorophore or a non-fluorescent molecule (Quencher). The most widely used quencher in fluorescent probes is 4-4-dimethylamino phenyl azo benzoic acid (Dabcyl). Which is incorporated at any position in the oligonucleotide sequence or labeled with nucleosides via long backbone amide-type or aliphatic linkers. It is used as a quencher in different hybridization-sensitive probes. Dabcyl has a maximal absorption range of 475 nm, and a quenching range of 400-550 nm. This property allows it to be widely used to burst a variety of common fluorescent dyes such as FAM, TET, and HEX. The quenching ability of Dabcyl has been intensively studied in many biological systems, such as monitoring enzymatic activity with nucleic acids or polypeptides.
Fig.1 Schematic illustration of formation and detecting principle of DRO probe. (Ning, et al., 2017)
CD BioGlyco provides Dabcyl doped into any position service in the sequence using a modifier. Due to the stabilization of the oligonucleotide synthesis process increasing the versatility of the Dabcyl molecule. The fluorescent fraction is sufficiently close for molecular beacons and in turn, allows quenching of the dye to a slightly broader spectrum.
Dabcyl is usually installed as overhang residues. In this arrangement, the Dabcyl part has significant mobility, which affects the accuracy of the distance/orientation measurements associated with fluorescence resonance energy transfer (FRET). Also, it limits the types of other conjugates that be prepared. To this end, our company's products simultaneously retain the ability to base pair through canonical hydrogen bonds, allowing a more flexible design and construction of molecular beacons based on the insertion of nuclear base fluorophores and quenchers into the stem sequence.
We provide double fluorophore-labeled oligonucleotides and two-color ratio triple fluorescent oligonucleotide probes. Using the FRET mechanism for fluorescence detection, the analysis method relies on a double fluorophore-labeled oligonucleotide. The homogeneous detection method is not only simple, with high sensitivity/specificity, but also no false positive signal and signal fluctuations. The special thing is the visual visualization of two different colors.
Fig.2 Classification of Dabcyl labeling sites. (CD BioGlyco)
CD BioGlyco is a leading company specializing in providing Fluorescent Dye Modification services for clients. We have reached perfect cooperation with clients in many countries to provide custom services for clients to meet their needs. If you are interested in our project, please feel free to contact us.
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