Applications of Pseudomonas aeruginosa Pel Polysaccharide

P. aeruginosa is a gram-negative, aerobic, non-spore-forming rod that can infect immune-competent and immune-compromised hosts, including people, with various diseases. Most bacteria in nature can cling to various surfaces and create biofilms. P. aeruginosa is a well-known producer of biofilms, and it contains several exopolysaccharides that are unique to biofilms, such as cellulose, alginate, Pel polysaccharide, and poly-N-acetylglucosamine (PNAG). Pel is a partly deacetylated cationic polysaccharide polymer comprising N-acetyl-d-glucosamine and N-acetyl-d-galactosamine. In nonmucoid strains, Pel is a crucial matrix component of the biofilm that is involved in both the start of surface attachment and the preservation of the biofilm's integrity.

Fig.1 Proposed model for biofilm architecture. (Geddes-McAlister, et al., 2019)

P. aeruginosa Pel Polysaccharide Production Services at CD BioGlyco

Pel, which comes in two forms—a high-molecular-weight cell-associated form and a low-molecular-weight secreted form—is a positively charged, partly deacetylated, -1,4 linked N-acetylgalactosamine (GalNAc) polymer made mostly of GalNAc-GalN repeat units. CD BioGlyco has the ability to purify various molecular weights to offer custom P. aeruginosa Pel polysaccharide production services.

The protein byproducts of the pelABCDEFG operon are necessary for the production of pel-dependent biofilms. Although a clear connection between Pel production and immunological compromise and/or virulence has not yet been demonstrated, it has been demonstrated that the secretion of this polysaccharide increases the in vitro resistance of P. aeruginosa to aminoglycoside substances. Different P. aeruginosa strains have varying abilities to create biofilms. This is an excellent tool to look at possible antibacterial uses, CD BioGlyco offers a Polysaccharide Vaccine Development Platform to support our clients' glycan-related research.

• Pel Polysaccharide Testing Service
  CD BioGlyco provides custom Pel polysaccharide production services, including extraction and purification of Pel from P. aeruginosa, and then use SDS-PAGE and Western blot tests of Pel polysaccharide. Besides these services, CD BioGlyco also provides MS-based and NMR-based analysis services for product quality control (QC). In addition, we also provide crystal violet and BACTH analysis, which is useful for researchers to study Pel polysaccharides.

Fig.2 QC of Pel polysaccharide at CD BioGlyco. (CD BioGlyco)

Advantages of Us

• We provide one-stop P. aeruginosa Pel polysaccharide production services for clients, from extraction and purification to quality control and characteristics.
• We provide different molecular weights of Pel polysaccharides according to the client's needs.
• We provide production progress on time and make each production step clear and trackable.
• We employ a good lab team that offers high-quality production outcomes.

CD BioGlyco provides high-quality P. aeruginosa Pel polysaccharide production services for our clients, if you have any questions or require detailed information, please feel free to contact us. We will reply in time and offer a quotation to you as soon as possible.

Reference:

1. Geddes-McAlister, J.; et al. Tasked with a challenging objective: Why do neutrophils fail to battle Pseudomonas aeruginosa biofilms. Pathogens. 2019, 8(4): 283.