Insect Cells: a System with Potential

CD BioGlyco prepares a recombinant baculovirus (rBV) by inserting a foreign gene into a plasmid transfer vector and then incorporating it into the baculovirus (BV) genome by homologous recombination. Many heterologous genes are efficiently expressed in this system by infecting insects or insect cell lines. We mainly provide two types of viral vectors: Autographa californica nuclear polyhedrosis virus (AcNPV) or Bombyx mori nuclear polyhedrosis virus (BmNPV). The products expressed in this system often have conformations similar to the native conformation with relatively high yields, and appropriate post-translational modification processes are also possible with the absence of endotoxin contamination.

• Selection of the host insects: Our experienced researchers help clients analyze the most suitable insect hosts, such as insect cell lines or insect larvae, to ensure that the target substances are expressed efficiently in the hosts.
• Viral vector construction: We offer efficient design and construct baculovirus vector services including appropriate promoters, messenger RNA (mRNA) regulatory elements, and target protein-coding sequences through multiple screenings.
• Optimization of infection conditions: According to the properties of the target material and the characteristics of the host cell line, the infection conditions are optimized, including the virus titer, the infection time, and the culture temperature.
• Purification and analysis: We provide various protein purification and analysis services such as affinity chromatography, gel electrophoresis, and mass spectrometry to ensure the high purity of the target protein and its structural and functional analysis.

Fig.1 Flow chart of small batch production of recombinant lectin in yeast system. (CD BioGlyco)

Advantages of Insect Cells Expression System

Tab.1 Advantages of yeast expression systems. (Demain, et al., 2009)

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Advantages of Us

• High expression level: Our professional talent team realizes a high level of protein expression, which reaches the natural expression level of insect cells.
• Large-capacity expression vectors: The baculovirus expression vectors we provide have a large capacity to accommodate large target protein genes.
• Strict process control: To verify the effect of the expression system, we set up appropriate control groups, such as an empty virus infection group and an empty vector transfection group.

CD BioGlyco is a professional manufacturer of lectin. We offer comprehensive Lectin Production services to clients worldwide. In addition, we also offer other Expression Systems including Bacteria, Yeast, and Mammalian Cells. Our trained staff offer special customized services according to client needs. Please feel free to contact us if you are interested in our service.