Stable Isotope Labeling Modification Service

Necessity of Stable Isotope Labeling

Isotopic labeling is a technique commonly used to trace the path of an element in chemical reactions and metabolism. Both tracer and flux studies benefit from the assistance of stable isotope labeling. It studies metabolic pathways by tracking naturally occurring and disease-indicative metabolites. Stable isotope-labeled internal standards can also be used in combination with mass spectrometry (MS) to elucidate the effects of exogenous substances or perturbations on the metabolome. Stable isotope labeling is also at the heart of nuclear magnetic resonance (NMR) and MS research on nucleic acid. Common stable isotopes include ²H, ¹³C, and ¹⁵N.

Stable Isotope Labeling Modification Service at CD BioGlyco

Nucleotides are the building blocks of all living organisms, making up the genetic units DNA and RNA. It plays a dominant role in the growth, development, reproduction, inheritance, and other important life phenomena of organisms. As nucleosides and nucleotides are more and more widely and deeply used in the field of biomedicine, people's demand for modified and labeled nucleosides and nucleotides is also increasing, and the quality requirements are also increasing. With the advanced Glyco™ Synthesis Platform, CD BioGlyco provides clients with high-quality nucleoside and nucleotide modification (Base, Sugar, and Phosphate Group-based Modification) and labeling services (Fluorescence Labeling, stable isotope labeling, and Radionuclide Labeling).

Stable isotope labeling service refers to the use of ²H, ¹³C, or ¹⁵N to replace the original 2H, 12C, and 14N atoms to synthesize nucleosides and nucleotides. Their relatively large molecular weight compared to standard molecules makes them powerful tools in protein quantification, protein structure analysis, and identification by MS or NMR. At CD BioGlyco, we provide our clients with isotopic labeling of a wide variety of nucleosides and nucleotides by chemical and enzymatic methods. The available stable isotopes include ²H, ¹³C, ¹⁵N, ¹⁸O, and ¹⁹F. Our labeled nucleosides and nucleotides include but are not limited to:

Fig.1 Labeled nucleosides and nucleotides. (CD BioGlyco)

Applications

• Modified nucleosides in nucleic acids are quantified by MS using a stable isotope-labeled internal standard.
• Nucleotide-specific labels provide the best opportunity for NMR to obtain structural information.
• The dynamic process of nucleic acid modification and de-modification is analyzed by nucleic acid isotope labeling-coupled MS (NAIL-MS).

Advantages of Us

• We provide clients with stable isotope labeling services of nucleosides and nucleotides with very competitive quality and price advantages.
• Choose the most suitable stable isotope and labeling strategy according to your application goals.
• Isotopic enrichment >98%, purity >95%.

At CD BioGlyco, our experienced chemists will work with you to design and prepare your stable isotope-labeled nucleoside and nucleotide products. Whether during production or after delivery, you could always feel free to contact us for more information.