Client Satisfaction Is the Driving Force of Our Work Related to Oligosaccharide Library Construction

CD BioGlyco has constructed a very complete Glycan Display Platform to provide support and services for glycan interaction studies and structure-function correlation studies of oligosaccharides. We have abundant oligosaccharide libraries to meet the needs of various analyses based on Traditional Glycan Display Arrays. To better serve our clients and escort them in every step of their research, we have developed a one-stop oligosaccharide library construction service based on chemical synthesis.

Oligosaccharide libraries play an important role in studying conformational relationships and revealing glycan functions. In addition to Natural Extraction and Enzymatic Synthesis, chemical synthesis is also a very effective method to construct oligosaccharide libraries. Through the advancement of technology and continuous research, we have successfully realized more effective and faster preparation of oligosaccharide collections. The process of oligosaccharide library preparation is as follows.

• Design and selection of oligos
  We determine the desired oligos to be included in the library by considering specific structural motifs, modifications, or known targets. The basis of selection is mainly based on the accumulated knowledge used to predict glycan-protein interactions, computational tools, etc.
• Selection of strategy and synthesis method
  Synthetic strategies and methods are determined based on the complexity, size, and modifications of the target oligosaccharides. We are proficient in one-pot glycosylation reactions, solid-phase glycosylation reactions, and other chemical reactions.
  • One-pot glycosylation reaction
    This reaction improves the overall efficiency of the synthesis by eliminating purification and subsequent processing and reducing the number of steps.
  • Solid-phase glycosylation reaction
    This reaction is an effective method for simplifying the reaction process and rapid synthesis of oligosaccharides. The reaction does not require the purification of reaction intermediates and is automated. The two main strategies currently used for it are acceptor binding and donor binding.
• Glycosylation
  Glycosylation reactions are performed to form glycosidic bonds between monosaccharide structural units. We couple an activated donor to an acceptor under appropriate reaction conditions. This process usually utilizes catalysts, activators, etc., to drive the reaction forward. During the reaction, we consider the effect of protecting groups, distal auxiliary groups, etc. on the glycosylation reaction.
• Purification and characterization
  The synthesized oligosaccharides are purified to remove reaction by-products, protecting groups, or other impurities resulting from the chemical synthesis process. We use techniques such as thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) for purification. The synthesized oligosaccharides are characterized using techniques such as NMR, mass spectrometry, and high-resolution analytical methods.

Fig.1 Process of chemical synthesis-based oligosaccharide library construction. (CD BioGlyco)

Publication Data

Applications

• Abundant libraries of oligosaccharides are used to assess the binding specificity of serum antibodies.
• Abundant libraries of oligosaccharides are used to reveal glycan functions in organisms, such as mediating cell adsorption, messaging, immune recognition, and pathogenic attack.
• Abundant oligosaccharide libraries are used to study the interactions and synthesis mechanisms of glycans with lipids and proteins, which is of great significance in the research of new drugs.

Advantages of Us

• We employ multiple chemical reactions to synthesize structurally diverse oligosaccharides to enrich our oligosaccharide library.
• We optimize our experimental processes to improve the overall efficiency of oligosaccharide synthesis.
• With our rich experience in oligosaccharides synthesis, we excel in tackling challenges such as stereoselective and regioselective areas in glycosidic bond formation.

CD BioGlyco is constantly refining and updating its technology and experimental procedures for oligosaccharide library construction. In addition to chemical synthesis, we also use a combination of chemical and enzymatic methods. Please feel free to contact us for more information about oligosaccharide chemical synthesis and library construction techniques. Our team is ready to answer your questions and serve you at any time.