At CD BioGlyco, we provide a cutting-edge service for traditional glycan display arrays. This specialized offering is designed to propel glycobiology research, biomarker discovery, and glycan-protein interaction studies to new heights. Our service encompasses three key components including oligosaccharide library construction, library immobilization, and glycan microarray assay, each executed with precision and expertise.
CD BioGlyco offers custom synthesis services for glycan libraries tailored for microarray chip applications, typically focusing on oligosaccharides. Our services encompass the design and synthesis of specialized glycan libraries to meet the specific requirements of microarray chip experiments.
This service includes the preparation of defined oligosaccharide structures, tailored to the client's needs, ensuring high quality and reproducibility. Our expertise in glycan synthesis enables the generation of custom glycan libraries suitable for diverse applications, such as glycan microarray assays, facilitating advanced studies in glycobiology, biomarker discovery, and glycan-protein interactions.
Besides these oligosaccharide library construction services, CD BioGlyco also utilizes technologies such as metabolic and genetic engineering to enable controlled editing of cell surface glycan display for cell-based glycan display array.
Our oligosaccharide library immobilization service offers a comprehensive solution for stable and reproducible glycan immobilization. Our expert team excels in coupling synthesized oligosaccharides to both N-hydroxysuccinimide-activated glass slides and epoxide-activated glass slides, ensuring robust attachment and preservation of glycan integrity. Moreover, we also provide expertise in depositing glycolipids and lipid-derivatized glycans onto nitrocellulose-coated slides, expanding the versatility of interactions.
Fig.1 Glycan immobilization and epitope recognition on a microarray. (Kiessling & Christopher, 2002)
CD BioGlyco's glycan microarray assay services offer a comprehensive exploration of glycan-protein interactions. These specialized assays provide in-depth insights into diverse glycan categories, allowing researchers to unravel the complexities of microbial surface structures, human milk oligosaccharides, sialylated glycans, glycosaminoglycans, and blood group antigens. The advantages include high-throughput screening, customization options, and significant contributions to glycobiology research and biomarker discovery. Choose CD BioGlyco for a transformative journey into the intricate world of glycans and their interactions!
Fig.2 The glycan microarray story from construction to applications. (Hyun, et al., 2017)
Technology: Carbohydrate microarrays, Glycan–protein interactions, One-step surface modification, Fluorescent detection
Journal: Accounts of Chemical Research
IF: 18.3
Published: 2017
Results: The presence of carbohydrates in glycoconjugates, such as glycoproteins and glycosphingolipids, plays a crucial role in physiological and pathological processes, engaging in interactions with glycan-binding proteins (GBPs). Carbohydrate microarrays, pioneered in 2002, facilitate the simultaneous analysis of multiple glycan–protein interactions, providing a powerful tool for studying glycans and GBPs. This technology enables rapid assessment of binding events, determination of affinities, substrate specificity of enzymes, pathogen detection, and identification of functional glycans influencing cellular responses, offering valuable insights into pathophysiological processes and potential therapeutic applications.
Fig.3 Glycans immobilized on a microarray (top) are displayed in a multivalent manner like cell surface glycans (bottom). (Hyun, et al., 2017)
At CD BioGlyco, we explore the forefront of glycobiology with our glycan microarray services. Join us in advancing research and unraveling the intricacies of glycan-mediated events with precision and efficiency. Please do not hesitate to contact us for more details!
References
