The Functions of Cyclic Adenosine Monophosphate (cAMP)

cAMP is a derivative of adenosine triphosphate. cAMP contains a 3', 5'-cyclic structure, which is formed by binding to the phosphate in nucleotides in the presence of ribose. cAMP is an intracellular signaling molecule responsible for directing cellular responses to extracellular signals, and serves as a ubiquitous second messenger that mediates a variety of biological processes, such as cell growth, inflammation, and metabolism. Meanwhile, it plays an important role in the regulation of sugar and lipid metabolism, nucleic acid, and protein synthesis. The status and role of cAMP in life activities determines that cAMP will soon be developed and applied, and consequently its production, so far, all known cAMP APIs for clinical use are synthesized by chemical method.

Fig.1 The structure of cAMP. (Gu, et al., 2021)

Cyclic Adenosine Monophosphate (cAMP) Production Service at CD BioGlyco

Although cAMP is widely found in organisms, its content is very small, generally only one-thousandth of ATP content. CD BioGlyco provides a variety of methods for the production of cAMP to provide clients with products with guaranteed quality. According to the different production purposes and uses, CD BioGlyco plans the most appropriate production route for you.

• Chemical synthesis

• With 5' AMP as the starting material, different individual organic reagents (such as dicyclohexyl carbon diimide DCC, tert-butanol potassium, triisopropyl phenyl sulfonyl chloride or triphenylphosphine, 2,2'-pyridine disulfide) were used as cyclization reagents, and anhydrous pyridine or ethylene glycol monomethyl ether was used as dissolving agents.
• ATP as starting material, started for catalytic cyclization using Ba (OH)₂ to produce cAMP.

• Bacterial production method

• We use microorganisms as raw material to produce cAMP through strain modification, metabolic regulation, fermentation optimization, and isolation and purification. Each step of the process was strictly controlled. We constantly optimize the quality of bacteria, reducing the proportion of impurities, and ensuring the maximum production, reachable 10.42 g/L.

• Yeast fermentation production method

• Saccharomyces cerevisiae cells accumulate extracellular cAMP and construct PKA activity level as the starting material for high cAMP strains. On this basis, the positive regulator of purine anabolic pathway Bas 1 p and Bas 2 p, optimize the medium components accordingly and greatly increase the extracellular cAMP level, reachable 5314.3 μmol/L.

Fig.2 The pathway of cAMP synthesis in Saccharomyces cerevisiae. (CD BioGlyco)

Applications of cAMP

• cAMP plays an irreplaceable role in pharmacological and clinical experimental studies.
• cAMP regulates hormone levels in mammals, such as promoting milk secretion from cows.
• cAMP improves the production performance of livestock and poultry.
• cAMP plays a key role in plant cell growth, disease resistance, and plant resistance to stress.

Advantages

• High yield, short reaction period, and high purity of the reaction products.
• During the production process, we use high separation and purification methods to ensure the high purity of cAMP.
• Our company uses renewable raw materials to produce cAMP pure products, which achieve sustainable utilization of resources, low pollution, and clean production.

CD BioGlyco possesses extensive expertise in the field of Carbohydrate Synthesis. We have accumulated a lot of experience in controlling process parameters to ensure high synthesis efficiency and product purity. Moreover, we provide high-quality GMP, FAD, FMN, NAD, NADP, and Guanosine Pentaphosphate (pppGpp) Production Services. Please feel free to contact us if you would like to know more information.