Evaluation of Metabolic Function Assays

CD BioGlyco provides highly accurate and reproducible mice body mass composition analysis services, multi-parameter real-time metabolic rate monitoring services, insulin tolerance tests services, and glucose tolerance tests services for mice.

Fig. 1 The glucose tolerance tests and insulin tolerance tests profile (Bruce CR, et al., 2021)

What We Do

Our Workflow

1. The mice cages are changed to clean ones the day before the experiment, and all mice fast for 16 h. For insulin resistance experiments, mice fast for 6 h during the fasting period, the drinking water was guaranteed to be normal.
2. Each mouse is weighed before the glucose tolerance test and insulin tolerance test.
3. Cut the mice tail 1-2 mm with scissors, squeeze the mice tail gently, and measure the fasting blood glucose of the mice.
4. After 30 min of acclimatization, the mice are ready to inject glucose or insulin.
5. The mice are gently picked up and injected with glucose or insulin following the protocol for the chosen dosing method.
6. Measure the blood glucose level of mice at 15 min, 30 min, 60 min, 90 min, and 120 min.

Applications

• To study the effects of metabolic pathway regulators on body composition, glucose tolerance, and insulin tolerance in tumor-bearing mice.
• To study the effect of cancer on body composition, and metabolic function in mice with metabolic diseases.

Notes

The glucose tolerance test we use is either 1.5 g/kg or 2 g/kg. In insulin resistance experiments, the amount of insulin we used was determined according to the age and sex of the mice, generally 0.5-1.2 U/kg. Both glucose and insulin we used were diluted to appropriate concentrations with normal saline.

CD BioGlyco provides mice mass composition analysis, metabolic function real-time monitoring, glucose tolerance test, and insulin tolerance test services. Our experienced teams of scientists, researchers, and technicians provide fast turnaround, high-quality services at competitive prices for worldwide customers. Our customers have direct access to our staff and prompt feedback on their inquiries. If you are interested in our services, please contact us for more details.

Reference:

1. Bruce CR, et al. Translating glucose tolerance data from mice to humans: Insights from stable isotope labelled glucose tolerance tests. Mol Metab. 2021 Nov; 53: 101281.