Glyconanoparticles are carriers that deliver specific molecules in intracellular sites and are used in various applications such as drug and gene delivery, bioassays for pathogens, protein detection, tissue engineering, tumor imaging, and targeting. They start by reaching the plasma membrane and enter the cell mainly through endocytosis. This is followed by a network of cellular endosomes mediating the guidance of glyconanoparticles to different cellular components or being released into the cytoplasm. Understanding the cellular uptake and intracellular trafficking is critical to the design of safe and efficient glyconanoparticles for these applications. CD BioGlyco has built the perfect GlycoNano™ Platform and provides industry-leading glyconanoparticle uptake and trafficking study service. We provide customized experimental design solutions based on specific research questions, enabling the study of unique aspects of glyconanoparticles.
Our glyconanoparticle uptake and trafficking research service helps advance innovation in the field of drug delivery and nanomedicine through innovative approaches and high-quality research. Specific services include the following:
We design and synthesize different types of glyconanoparticles (Carbohydrate-based Nanoparticles, Gold Glyconanoparticles, etc.). The entire synthesis and purification process has been optimized to ensure the high quality of glyconanoparticles. Cellular uptake of glyconanoparticles is a complex process involving highly regulated mechanisms (endocytosis-based uptake pathway and direct cellular entry) and is influenced by a variety of factors, e.g. particle size, particle morphology, surface modifications, charge, cell type, etc. We tailor the physicochemical properties of glyconanoparticles to optimize their cellular uptake, targeting, and intracellular transport.
This process includes the study of the Physicochemical Properties, Interactions, Stability, biocompatibility, Safety, etc., of glyconanoparticles. We combine a variety of assay techniques to fully analyze them.
Dynamic light scattering (DLS)
Scanning electron microscopy (SEM)
Transmission electron microscopy (TEM)
Nuclear magnetic resonance (NMR)
X-ray diffraction (XRD)
Surface plasmon resonance (SPR)
Other inspection techniques
Based on our extensive experience in Preclinical Studies and biological characterization of glyconanoparticles, we use bioinformatics methods, SEM, TEM, flow cytometry, laser confocal microscopy, and other techniques to simulate and probe the uptake and intracellular trafficking of glyconanoparticles. Various cell lines (cancer cell lines, primary cells, etc.) are used to assess the uptake, distribution, and subsequent transport pathways of glyconanoparticles. In addition to this, various animal models are utilized for relevant in vivo studies.
Molecular dynamics simulation
Real-time observation of cellular uptake
Cellular uptake rate assay
Uptake mechanism and pathway analysis
Our glyconanoparticle uptake and trafficking research service employs a multidisciplinary approach combining chemistry, biology, and imaging techniques with the aim of gaining insights into the behavior of glyconanoparticles in biological systems, and ultimately advancing their application in nanomedicine and targeted therapies.
Technology: Dynamic confocal imaging
Journal: Nanoscale Research Letters
IF: 3.159
Published: 2018
Results: Many diseases have utilized nanoparticles as a tool for their treatment and diagnosis. This paper describes the different possible uptake pathways and intracellular transport pathways of nanoparticles. In addition, the influence of physicochemical properties such as shape, size, and charge of nanoparticles in determining the uptake mechanism and biological function of nanoparticles is discussed. Understanding the correlation between the physicochemical properties of nanoparticles and their cellular uptake mechanism provides theoretical support for the good design of functional glyconanoparticles for biomedical applications.
Fig.1 Physicochemical properties affecting cellular uptake of nanoparticles. (Foroozandeh & Aziz, 2018)
CD BioGlyco provides a glyconanoparticle uptake and trafficking research service that exceeds client expectations. Our comprehensive expertise and state-of-the-art technology will support your research and drive your project to success. If you have more specific questions or need more information, please feel free to contact us for a consultation! We are prepared to discuss your specific research needs, study design, and other expectations at any time.
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