Development of Gene Editing Technology

People's ability to read large amounts of sequence information has been rapidly improved with the completion of the Human Genome Project and the development of high-throughput sequencing technology. In recent years, advances in gene editing technology have greatly improved people's ability to rewrite genetic sequences in organisms. It allows researchers to artificially edit gene transcripts or genome sequences to change the sequence, expression, or function of target genes or regulatory elements. This technology has a wide impact on many fields such as disease treatment and new drug development.

Homologous recombination (HR) is the earliest gene editing technology discovered. It realizes gene editing by introducing foreign genes and exchanging between homologous sequences, but the efficiency is very low. Later, the gene editing technology of nuclease appeared, which can modify specific genes without introducing foreign genes.

Fig.1 The homing process. (Barzel, et al., 2011)

Homing Endonuclease-based Glycogene Editing Technology at CD BioGlyco

In the field of glycobiology, simple and efficient precise gene editing techniques are used to dissect the glycosylation pathways and biological functions of complex carbohydrates. It greatly facilitates access and a broader understanding of carbohydrates. At CD BioGlyco, we have developed high-quality Glycogene Editing Services to help global companies, universities, and research institutes solve their difficulties and problems in gene editing.

Fig.2 Technologies for glycogene editing. (CD BioGlyco)

Homing endonucleases are the first discovered precise genome editing tools based on nucleases, a family of endonucleases encoded by mobile genetic elements. It uses a homing endonuclease to cause a double-stranded break (DSB) near the target site of the genome, activates the damage repair mechanism to participate in the repair of the break, and then improves the efficiency of gene editing. Homing endonucleases are generally very specific. Currently, a common homing endonuclease targeting glycogenes is fucosyltransferase 8 (FUT8). We achieve disruption, deletion, insertion, correction, mutation, and labeling of glycogenes by generating site-specific DSBs or nicks through highly active and specific homing endonucleases.

Applications

• We inhibit or activate target gene expression through gene editing techniques.
• For the construction of cell animal models, the gene editing technique directly modifies the target gene at the embryonic level, which greatly improves production efficiency.
• The application prospect of gene editing technology in the medical field has been discovered. Using gene editing technology to study disease-related glycogenes.

Advantages of Us

• We have developed an advanced Glycogenomics Platform and provide a series of services including gene editing, delivery, and expression analysis.
• We perform disruption, deletion, insertion, correction, mutation, and labeling of glycogenes.
• Highly active and specific glycogene editing technique.
• The team led by doctors designs exclusive customized solutions according to your requirements and guides and follows up the operation of the project throughout the process.

CD BioGlyco has an advanced experimental platform and an experienced team of scientists and is committed to providing clients with the highest-quality glycogene editing services. We have been recognized by scientists from many countries. If you happen to need a glycogene editing service, please feel free to contact us, and our sales will communicate with you as soon as possible.

References:

1. Steentoft, C.; et al. Precision genome editing: a small revolution for glycobiology. Glycobiology. 2014, 24(8): 663-680.
2. Barzel, A.; et al. Native homing endonucleases can target conserved genes in humans and in animal models. Nucleic acids research. 2011, 39(15): 6646-6659.