Technologies for Glycogene Editing

Principles of Gene Editing

Gene editing is to make heritable changes by inserting, replacing, deleting, and other targeted modifications or changes of the gene sequence in the gene fragments of organisms. It changes the composition and structure of genes in a directional way, which has the advantages of efficient, controllable, and directional operation.

Current gene editing technologies include homing endonucleases (HEs), zinc finger nuclease (ZFNs), transcription activator-like effector nuclease (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR), etc. They use nucleases to specifically cut deoxyribonucleic acid (DNA) to form double-stranded breaks (DSB) and carry out the purpose of repairing, to achieve the purpose of modifying the gene fragment. These technologies provide unprecedented convenience and speed for gene editing and have attracted extensive attention from researchers all over the world.

Fig.1 Mechanism of ZFN, TALEN, and CRISPR. (Chen, et al., 2014)

Technologies for Glycogene Editing at CD BioGlyco

At CD BioGlyco, we have established a best-in-class Glycogenomic Platform, providing clients with services of Glycogene Editing, Glycogene Delivery, Glycogene Expression Profiling, and Cancer Glycogene Discovery Service. A major challenge in the field of glycobiology is to convert complex glycan structures and biosynthetic pathways into more predictable and controllable networks of glycogenes, glycoenzymes, and glycostructures. Nuclease-based precise genome editing technology genetically dissects the glycosylation pathways and biological functions of complex carbohydrates, triggering a revolution in the field of glycobiology.

• HE-based Glycogene Editing Technology
  HE is the first nuclease-based precise genome editing tool discovered. Through this editing technology, we provide clients with glycogene editing services such as fucosyltransferase 8 (FUT 8).
• ZFN-based Glycogene Editing Technology
  ZFNs are non-native chimeric proteins consisting of zinc finger DNA-binding domains and nonspecific DNA nucleases. This editing technology is used to rapidly edit genes in a variety of species.
• TALEN-based Glycogene Editing Technology
  TALENs use FokI nuclease to make DSBs. The advantage of TALEN is that it can be used as a target to construct a specific TALEN nuclease for any nucleotide sequence.
• CRISPR/Cas-based Glycogene Editing Technology
  RNA-guided CRISPR/Cas systems simultaneously deliver multiple single guide ribonucleic acids (sgRNAs) targeting different loci for multiplexed analysis, providing possibilities for genomic analysis.

Fig.2 Basic flowchart of CRISPR-Cas9-mediated genome modification. (CD BioGlyco)

• Base Editing-based Glycogene Editing Technology
  Base editing is a CRISPR-Cas9-based genome editing technology that has the advantage of chemically introducing point mutations in DNA without generating DSBs.
• Prime Editing-based Glycogene Editing Technology
  Prime editing is a "search and replace" genome editing technique that enables any possible base substitution, insertion, or deletion without the need for a DSB or donor DNA template.

Our technologies for glycogene editing include HEs, ZFNs, TALENs, CRISPR, base editing, and prime editing. With these technologies, we quickly and accurately edit the genome of model organisms, such as pigs that knock out α1,3-galactosyltransferase to eliminate xenotransplantation barriers and edit endogenous genes in almost any cell line. In addition, we also use gene editing technology to explore the role of glycosylation genes and dissect specific structure-function relationships.

Advantages of Us

• We have developed a first-class glycogenomics platform to provide clients with a series of services such as glycogene editing, delivery, expression profiling, and glycogene development in cancer.
• We help clients explore glycogenes, glycoenzymes, and glycostructure networks through fast and accurate precise gene editing technologies.
• Not only do we use DSB-based gene editing technology, but we also apply base editing and prime editing without DSB.
• Our professional team will design a customized experimental plan according to your needs.

CD BioGlyco is committed to providing clients with the highest quality and most comprehensive glycogenomics services. In recent years, we have helped many clients from enterprises, universities, and research institutes solve their difficulties and problems in gene editing. If you are interested in our services, please feel free to contact us for more information.

Reference:

1. Chen, L.; et al. Advances in genome editing technology and its promising application in evolutionary and ecological studies. Gigascience. 2014, 3(1): 2047.