As a modification method, 2'-MOE is often used to decorate oligonucleotides, which can increase the resistance of nuclease. When 2'-OH functionality is not needed, the internucleotide phosphodiester linkages can be phosphorothioated in the oligonucleotide for better protection against nuclease attacks. The decoration at this site keeps enhanced preorganization of the sugar's A-type backbone conformation. The modification also provides chemical stability as it prevents 2'-OH-mediated chain cleavage. Additionally, it increases affinity, reduces toxicity, minimizes nonspecific protein linking, and extends half-life. MOE ASOs have demonstrated excellent pharmacokinetic properties and potent pharmacological activity.
CD BioGlyco provides polytypes of modification services based on oligonucleotides, including Phosphorylation Modification, Suger Ring Modification, Fluorophore Modification, and base modification services.
As a method of base modification, 2'-MOE includes four aspects, namely 2-MethoxyEthoxy A, 2-MethoxyEthoxy T, 2-MethoxyEthoxy G, and 2-MethoxyEthoxy MeC. The following services can be provided.
CD BioGlyco provides customized 2'-MOE modification for specific RNA sequences according to the customer's request, including determining the modification sites, the number of modifications, and other modification parameters.
CD BioGlyco synthesizes nucleotides with 2'-MOE modification based on customer-provided sequences, which can be used for further experiments and research.
Analyze the quality and purity of 2'-O-MOE modified RNA samples using various analytical techniques such as high-performance liquid chromatography (HPLC), LC-MS, and capillary electrophoresis, etc.
Simultaneously, CD BioGlyco also offers other oligonucleotide-based bases modification services including Affinity-plus Base, Inosine, 2'-O-methyl RNA Bases, and Fluoro Bases, and oligonucleotide synthesis and purification services.
Fig.1 Oligonucleotide-based 2'-MOE bases modification services. (CD BioGlyco)
CD BioGlyco is committed to offering high-quality 2'-MOE modification service for the oligonucleotide. Our technical team will continuously keep track of the project process, and provide instant guidance to our customers. Please feel free to contact us as soon as possible if you would like to obtain our base modification services.
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