Applications of 2'-MOE Base Modification

As a modification method, 2'-MOE is often used to decorate oligonucleotides, which can increase the resistance of nuclease. When 2'-OH functionality is not needed, the internucleotide phosphodiester linkages can be phosphorothioated in the oligonucleotide for better protection against nuclease attacks. The decoration at this site keeps enhanced preorganization of the sugar's A-type backbone conformation. The modification also provides chemical stability as it prevents 2'-OH-mediated chain cleavage. Additionally, it increases affinity, reduces toxicity, minimizes nonspecific protein linking, and extends half-life. MOE ASOs have demonstrated excellent pharmacokinetic properties and potent pharmacological activity.

Oligonucleotide-based 2'-MOE Bases Modification Services at CD BioGlyco

CD BioGlyco provides polytypes of modification services based on oligonucleotides, including Phosphorylation Modification, Suger Ring Modification, Fluorophore Modification, and base modification services.

As a method of base modification, 2'-MOE includes four aspects, namely 2-MethoxyEthoxy A, 2-MethoxyEthoxy T, 2-MethoxyEthoxy G, and 2-MethoxyEthoxy MeC. The following services can be provided.

Other Oligonucleotide-based Bases Modification Service at CD BioGlyco

Simultaneously, CD BioGlyco also offers other oligonucleotide-based bases modification services including Affinity-plus Base, Inosine, 2'-O-methyl RNA Bases, and Fluoro Bases, and oligonucleotide synthesis and purification services.

Fig.1 Oligonucleotide-based 2'-MOE bases modification services. (CD BioGlyco)

Applications

• RNA interference: 2'-MOE modification can be applied in the synthesis of small interfering RNA (siRNA), which is a tool to suppress gene expression. Introducing 2'-MOE modification on the single-stranded RNA of siRNA enhances its targeting efficiency and stability.
• Gene expression: 2'-MOE base chemical modification of oligonucleotides can be used as reagents to regulate gene expression in animal models and cell cultures.
• Drug development: 2'-MOE modification can extend the half-life of medicine, and improve the binding ability of the medicine and the target. 2'-MOE modification can also be used in the synthesis of modified mRNA for therapeutic. Modified mRNA can be utilized in protein expression and gene therapy fields, and incorporating 2'-MOE modification increases mRNA stability, improves translation efficiency, and reduces immunogenicity.

Advantages

• CD BioGlyco has many years of experience in oligonucleotide research and can synthesize and modify various complex oligonucleotides.
• Our laboratory is equipped with advanced synthesis and purification experimental platforms to ensure the high quality of the final product.
• The synthesis specifications we provide are flexible and can be customized in batches according to customer needs.

CD BioGlyco is committed to offering high-quality 2'-MOE modification service for the oligonucleotide. Our technical team will continuously keep track of the project process, and provide instant guidance to our customers. Please feel free to contact us as soon as possible if you would like to obtain our base modification services.