Introduction of 2'-O-methyl RNA Bases

2'-O-methyl RNA bases modification is a method of chemical modification specifically targeted towards oligonucleotides. In tRNA and other RNAs, 2'-O-methyl RNA is a naturally present modification, which can be part of custom-designed oligonucleotide configurations, where a 2'-O-methyl group is added to the ribose sugar of the oligonucleotide (Fig.1), altering its chemical structure and thereby changing its properties and functions. To include 2'-O-methyl modifications in an RNA sequence, simply place a lowercase "m" in front of the base; for example, mA, mG, mC, mU.

Compared to unmodified oligonucleotides, 2'-O-methyl modification is used to enhance the stability of oligonucleotides, prevent degradation by nucleases, and improve biological activity within cells. It also reduces nonspecific protein binding and increases the affinity and specificity of oligonucleotides for target RNA sequences. 2'-O-methyl modification finds widespread applications in various biomedical research and application fields, providing a useful tool for regulating the activity, selectivity, and stability of RNA.

Fig.1 Process of the 2'-O-methylation. (Yang, et al., 2018)

Oligonucleotide-based 2'-O-Methyl RNA Bases Synthesis Services at CD BioGlyco

• Primer design and synthesis

CD BioGlyco designs and synthesizes primers with 2'-O-Methyl RNA base modifications, which can be applied to molecular biology techniques.

• Custom synthesis

CD BioGlyco offers a customized synthesis of 2'-O-methyl RNA bases based on customer requirements and target applications, which contains the synthesis of 2'-O-Methyl RNA of a specific length, base combination, and modification pattern.

• Large-scale synthesis

We offer scale-up synthesis services for 2'-O-Methyl RNA base modifications when larger quantities are required.

Oligonucleotide-based 2'-O-Methyl RNA Bases Modification Services at CD BioGlyco

CD BioGlyco has extensive expertise in oligonucleotide production and chemical modification. With years of experience in custom oligonucleotide modification, CD BioGlyco is confident in providing comprehensive oligonucleotide modification services to clients worldwide, which include 2'-O-methyl RNA Bases Modification, Affinity-plus Base, Inosine, 2'-O-methoxy-ethyl Bases (2'-MOE), and Fluoro Bases.

Oligonucleotide-based 2'-O-Methyl RNA Bases Purification Services at CD BioGlyco

• CD BioGlyco performs appropriate purification techniques to obtain highly pure 2'-O-methyl RNA products, such as reverse-phase high-performance liquid chromatography (RP-HPLC).
• Quality analysis and purity assessment are also measured to validate the structure and purity of produce by mass spectrometry analysis and nuclear magnetic resonance (NMR).

These services aim to assist customers in obtaining target oligonucleotides, the specific service details and processes can be tailored according to customer needs.

Fig.2 Oligonucleotide-based 2'-O-Methyl RNA synthesis, bases modification and purification services. (CD BioGlyco)

Applications

• RNA interference (RNAi): 2'-O-Methyl RNA bases modification may enhance the stability and resistance to nuclease degradation of oligonucleotides, leading to improved RNAi effectiveness.
• Aptamer technology: 2'-O-Methyl RNA bases modification may improve the binding specificity and affinity of oligonucleotides toward target molecules, making it valuable for aptamer technology.
• Gene silencing: 2'-O-Methyl RNA bases modification may alter affinity and specificity for oligonucleotides and target RNA sequences, enabling gene silencing.
• Drug research: 2'-O-Methyl RNA bases modification can improve the in vivo stability of oligonucleotides, providing methods and strategies for oligonucleotide-based drug development.

Advantages

• Professional team: High-level scientists can apply the latest biotechnology methods and tools to provide high-quality and efficient 2'-O-Methyl RNA-based modification services.
• Customized services: We provide flexibly customized base modification services according to the needs of customers.
• Facilities and equipment: We provide accurate and reliable experimental results and products, with advanced laboratory facilities and biotechnology equipment.

CD BioGlyco offers technical support and consultation services regarding 2'-O-Methyl RNA base modifications and synthesis. Our technical team will continuously keep track of the project process, and provide instant guidance to customers. Please feel free to contact us in time if you need oligonucleotide modification services, we are your first choice.

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