Trisialoganglioside (GT) Analysis Service

CD BioGlyco is a world-leading independent biotechnology company, focusing on high-quality Lipidomics Analysis Services. We have a professional analytical platform with multiple world-leading technologies to help clients comprehensively analyze trisialoganglioside (GT).

Overview of GT

GT participates in cellular proliferation, differentiation, adhesion, signal transduction, cell-to-cell interactions, tumorigenesis, and metastasis. GT1 gangliosides bind to myelin, the insulating layer that wraps around nerves, and thus connects it with the neuron. GT2 is associated with tumors. It acts as an immediate precursor to the more complex GT1 gangliosides. GT3 is elevated in the human retina, suggesting that it may play specific roles in the structure and function of the tissue. It also acts as a precursor to more complex gangliosides.

Fig.1 Classification of gangliosides. (Liu, et al., 2018)

GT Analysis Service at CD BioGlyco

CD BioGlyco provides gas-liquid chromatography (GLC) and mass spectrometric technologies to analyze GT. Taking bovine adrenal medulla tissue as an example, we isolate and characterize it through the following procedures.

• Isolation of GT

Homogenize bovine adrenal medulla tissue in cold acetone. Check the purity of the isolated gangliosides by thin-layer chromatography (TLC) using the appropriate solvent system.

• Analytical procedures

Compositional analysis is performed by gas-liquid chromatography, mainly analyzing neutral sugars, sialic acid, fatty acids, and long-chain bases.

• Neuraminidase digestion

The enzymatic reaction is carried out using neuraminidase. Each sample under different hydrolysis conditions is dried under nitrogen flow and the salts are removed by column chromatography.

• Permethylation study

Purification of permethylated gangliosides is achieved by TLC and a chloroform-methanol-hexane solvent system. Trimethylsiloxy derivatives of methanol products are analyzed by GLC and GLC-mass spectrometry (MS).

Advantages of Us

• Expert consultation on best practices for sample collection, pre-processing, and storage to preserve sample integrity.
• The MS-based approach we provide is a solid analytical platform.
• Cutting-edge technology and professional research team.
• Different experimental programs for different requirements.
• Comprehensive and reliable after-sales service.

CD BioGlyco has been working on glycoscience for many years. We have developed systematic solutions to provide clients with Ganglioside Analysis. GLC and MS technologies are powerful tools for analyzing GT. If you are interested in our services, please contact us for more details without any hesitation.

Reference

1. Liu, J.; et al. Ganglioside GD3 synthase (GD3S), a novel cancer drug target. Acta Pharmaceutica Sinica B. 2018, 8(5): 713-720.