Cell Surface Blood Group Antigens Glycoengineering Service

What Is Blood Group Antigen?

Blood group antigens usually appear on the surface of red blood cells, which play vital physiological and immunological roles in organisms, including two main blood types: ABO and Rh. The antigens of the ABO blood group are sugars, while Rh antigens are proteins. Moreover, the membrane of red blood cells also includes proteins. The engineering of sugar chains-based cell surfaces may realize the synthesis, modification, and regulation of blood group antigens, thereby affecting related biological functions of cells, which is applied in fields such as basic research, biomedical research, and clinical applications.

Fig.1 Blood group antigen types-based red blood cell surface. (Dean, 2005)

Cell Surface Blood Group Antigens Glycoengineering Services at CD BioGlyco

CD BioGlyco provides a variety of cell surfaces-based blood group antigens glycoengineering services.

Type O

CD BioGlyco provides high-quality blood type O sugar chain-based cell surface glycoengineering services. Since the allelic expression product of individuals with type O blood lacks one nucleotide, the resulting protein is a product without enzymatic activity.

Type A

CD BioGlyco provides professional blood type A sugar chain-based cell surface glycoengineering services. The difference between blood group antigens lies in the terminal residues of the sugar chain, the end of the sugar chain of type A antigen is N-acetyl galactose.

Type B

CD BioGlyco provides mature blood type B sugar chain-based cell surface glycoengineering services. The B allele encodes GTB, which transfers a galactose residue to the H antigen to obtain the B antigen, thus giving blood type B.

Type AB

CD BioGlyco provides reliable blood type AB sugar chain-based cell surface glycoengineering services. The human ABO(H) blood group A and B antigens are synthesized from specific GTA and GTB, an individual with genes for both enzymes or cis-acting forms of the GTA or GTB have type AB blood.

Type Rh

CD BioGlyco provides excellent blood type Rh sugar chain-based cell surface glycoengineering services. The Rh system is composed of numerous antigens located in variant forms of theRhD and RhCE proteins, which form a core complex with glycosylation homologs (RhAG) in the erythrocyte membrane and are expressed only in their presence.

CD BioGlyco has an excellent glycoengineering service platform and we provide professional services.

Directed introduction of blood group antigens-based cell surface

CD BioGlyco introduces specific blood group antigens-based cell surfaces via various techniques.

Gene engineering
Viral vector-mediated transfection
Liposome-mediated transfection

Modification and regulation of blood group antigen-based cell surface

The blood group antigens-based cell surfaces are modified using targeted enzymatic reactions and chemical modifications. Regulating the expression levels of related enzymes controls the type, quantity, and structure of blood group antigen modifications-based cell surface.

Structure optimization of blood group antigen

Through methods such as enzyme engineering, substrate engineering, or chemical modification, the glycosyl structure of the blood group antigen is changed to enhance its immunogenicity, stability, or ability to interact with other molecules.

Analysis of blood group antigen

Qualitative and quantitative analyses of cells modified with blood group antigens are performed to assess the levels and characteristics of expression.

Immunostaining
Immunoprecipitation
Flow cytometry
Mass spectrometry

Development of biomarker for blood group antigen

Using blood group antigen sugars-based cell surfaces as biomarkers, CD BioGlyco provides the development of new molecular probes, antibodies, and biosensors.

BlCell surface blood group antigens glycoengineering services. Fig.2 Cell surface blood group antigens glycoengineering services. (CD BioGlyco)

Applications

Blood group antigen-modified cell models are used for drug screening, drug efficacy evaluation, and drug tolerance studies to understand the impact of different blood group antigens on drug response and therapeutic effects.
By regulating blood group antigens, the process of blood group identification and blood transfusion matching are simulated in vitro, providing relevant research support for immune diagnosis and blood transfusion safety.
Specific blood group antigens are introduced into tumor marker molecules to improve their selectivity and specificity, which provide new targets and strategies for tumor diagnosis and treatment.

Advantages

Our scientists have extensive professional knowledge, technology, and experience to efficiently provide cell surface blood group antigen glycoengineering services.
CD BioGlyco provides custom glycoengineering solutions according to the specific needs of clients, such as: synthesizing the specific blood group antigen and regulating its expression level.
Advanced analysis technologies are used for accurate structural analysis and confirmation of blood group antigens.

CD BioGlyco provides one-stop cell surface blood group antigens glycoengineering services, including blood type O, type A, type B, type AB, and type Rh. Please feel free to contact us if you are interested in cell surface blood group antigen services.

References

Dean, L. Blood groups and red cell antigens. Bethesda (MD): National Center for Biotechnology Information (US). 2005, Chapter 5, The ABO blood group.
Zhang, H.; et al. Typing of blood-group antigens on neutral oligosaccharides by negative-ion electrospray ionization tandem mass spectrometry. Analytical Chemistry. 2013, 85(12): 5940-5949.

This service is for Research Use Only, not intended for any clinical use.