Overview

Fluorescent glyconanoparticles combine fluorescent properties with the specific recognition of glycan molecules, making them highly specific and sensitive, and showing great potential for biomedical, chemical sensing, and materials science applications. Their fluorescent properties allow for effective detection and imaging at the microscopic level, while the presence of sugar molecules gives them the ability to recognize specific biomolecules or cells.

Unveiling the Secret of Fluorescent Glyconanoparticle Production

Glyconanotechnology utilizes the unique properties of nanomaterials to study and manipulate the structure and function of saccharides. CD BioGlyco has advanced GlycoNano™ Platform and has extensive research experience in the field of glyconanomics. We offer a wide range of Glyconanoparticle Production Services to our clients, in which we produce nanoparticles including but not limited to Carbohydrate-based Nanoparticle, Gold Glyconanoparticle, Silver Glyconanoparticle, Magnetic Glyconanoparticle, Quantum Dot (QD), and fluorescent glyconanoparticle.

Workflow 

Synthesis of Functionalized QDs

First, we synthesize water-soluble CdS QDs using mercaptopropionic acid (MPA) as a stabilizer. The MPA-capped CdS QD solution is mixed with EDC and 3-aminophenylboronic acid (APBA) for the conjugation reaction. After shaking the reaction for 3 h at room temperature away from light, unbound APBA is removed using ultrafiltration to obtain APBA-functionalized QDs (APBA QDs). APBA is covalently bound to the CdS QDs during the synthesis process, and the successful synthesis of the functionalized QDs provided the necessary recognition capability for the subsequent synthesis of glyconanoparticles, enabling the sugar nanoparticles to enhance the signal by binding to the functionalized QDs.

Synthesis of Fluorescent Markers

APBA and FITC are reacted under light-free conditions for 24h to obtain APBA-functionalized FITC. After the reaction, purification by silica gel column chromatography is performed to obtain APBA FITC.

Synthesis of Fluorescent Glyconanoparticles

We prepare PSA gold nanoparticles (AuNPs) by reducing HAuCl₄ with the help of citric acid and stabilizing it with polyvinyl alcohol (PSA). HAuCl₄ and PSA are mixed and heated, followed by the addition of citric acid and stirring to obtain a clear red glyconanoparticle solution. After adding APBA QDs to the PSA AuNPs solution, a change in the color of the solution (from red to purple) is observed, which indicates that the APBA QDs have undergone a cross-linking reaction with the PSA AuNPs through their PBA groups to form aggregates.

Blocking Non-specific Adsorption

In our experiments, we use 1% bovine serum albumin (BSA) as a sealing agent, which can effectively block non-specific adsorption. After cell culture, the microtiter plate is first closed with BSA, which can reduce the non-specific binding between the probe and the surface of the microtiter plate, thus improving the specificity of the assay.

Applications

• In drug development, it can be used as a drug carrier for targeted delivery, improving therapeutic efficacy and reducing side effects.
• It can be effectively used to build chemical sensors with high sensitivity, capable of detecting trace amounts of substances present in the environment with extreme precision.
• In the biomedical field, it can be used for cell imaging and tracking to help study the physiological and pathological processes of cells.

Advantages

• We precisely modulate the size, shape, and chemical composition of the nanoparticles to optimize their fluorescence properties and the binding efficiency of the sugar molecules.
• Our manufacturing technology enables large-scale production and meets the needs from laboratory research to real-world applications.
• In the production process, conditions are carefully optimized so that the nanoparticles maintain a stable structure and excellent performance under a wide range of environmental conditions.

Publication Data

Frequently Asked Questions

• How about the biocompatible of fluorescent glyconanoparticles?
  They tend to exhibit better biocompatibility if the core material chosen has low toxicity and the surface is suitably modified, e.g., covered with a hydrophilic polymer or a biocompatible molecule. With our rational design and optimization, fluorescent sugar nanoparticles can achieve good biocompatibility, thus providing the possibility of their application in biomedical fields.
• How is the stability of fluorescent glyconanoparticles in vivo?
  Smaller-sized particles may be more readily cleared by the kidneys, whereas larger-sized particles may remain in the body for longer periods, but may also face more phagocytosis by macrophages. Suitable surface coatings can enhance stability by reducing non-specific interactions with biomolecules in vivo and decreasing the probability of recognition and clearance by the immune system. With careful design and optimization, such as selecting appropriate materials, precisely controlling the particle size, and optimizing the surface modification, Fluorescent glyconanoparticles can maintain a certain degree of stability in vivo to achieve their intended functions, such as prolonged imaging, drug delivery, and so on.

CD BioGlyco is committed to offering first-class fluorescent glyconanoparticle production services. We possess a group of highly proficient experts and cutting-edge facilities to guarantee the efficient and accurate synthesis of fluorescent glyconanoparticles. If you need any assistance regarding our services, please feel free to contact us!