Overview

As a leading supplier in the field of microarrays, CD BioGlyco is committed to designing and developing various effective biological microarray products for non-clinical research and providing corresponding testing services. Our microarray test platform provides a full range of analysis and determination services for the study of lectin-related carbohydrates and glycoproteins to help our global customers conduct relevant research and reduce research costs.

Lectin Binding Protein Profiling

The lectin microarray consists of a set of lectins with unique glycan-binding properties printed on a solid support. The emergence of high-throughput glycomics technology enables lectin microarrays to simultaneously observe a variety of unique binding interactions, which has become the main method for studying glycosylation of entire samples. The most significant advantage of the lectin microarray over the glycan profile analysis method is that it only requires minimal sample preparation without releasing glycans, and can simultaneously quantitatively analyze different types of glycans on the complete biological structure. And these glycans can provide a global view of glycosylation status in their local environment. Lectin microarrays can be used to analyze precise glycosylation in various cells during cell development and differentiation to analyze cellular processes, including cell development and differentiation, cell-to-cell communication, pathogen host identification, and cell surface biomarker identification. In addition, it can quickly assess the real-time changes of surface carbohydrate epitopes and examine the dynamic effects of responses to external stimuli such as bacterial sugars. It can also be applied to the development of specific disease diagnostic biomarkers in clinical related research.

Key Technologies

Our service is built upon the powerful principle of using immobilized lectins to analyze the glycan structures present in a sample. Lectins are a class of proteins that bind specifically to certain sugar structures, acting as highly specific probes for different glycan moieties. Our core technology utilizes a high-density microarray platform where an extensive panel of lectins is spotted onto a solid surface. When a biological sample, such as serum or cell lysate, is applied to the microarray, the glycoproteins present bind to their specific lectin partners. This interaction is then detected and quantified, providing a detailed glycosylation profile of the sample. To complement this, we can integrate the microarray analysis with advanced mass spectrometry (MS) to achieve an even deeper level of detail. This combined approach allows for not only a broad, high-throughput overview of the glycome but also the precise structural identification of individual glycans and their protein-binding sites.

Lectin Arrayed, Precision Delivered: Your Glycan Code Deciphered.

Our microarray detection platform based on lectin microarray technology provides lectin conjugate analysis services. Our service process includes sample preparation, chemical labeling, microarray hybridization, and data analysis and processing. Our service can obtain high-throughput N or O glycan analysis maps from cell lysates or body fluids through lectin microarrays to quantify glycoprotein profiles. It can also be combined with mass spectrometry and other technologies to accurately analyze the structure and content of sugar chains or related glycoproteins bound by lectins. Our microarray service can be used to analyze the information of lectin-binding proteins as well as glycoprotein analysis. Lectin microarrays can be based on precise changes in protein sugar patterns, with minimal sample preparation without the need to release glycans, to ensure the true state of protein glycosylation in the tester's clinical samples. This helps to screen early patients with different types of cancers and correctly reflect some of the molecules in the body fluids of healthy, benign lesions and cancer that can be used as potential biomarkers. These characteristics make lectin microarrays very important for the analysis of precious clinical samples.

Workflow

• Sample Preparation and Glycoprotein Labeling

The process begins with the preparation of your biological samples (e.g., cell lysates or serum). Glycoproteins within the sample are then labeled, typically with a fluorescent dye, to enable detection on the microarray surface. This step ensures a strong and clear signal for subsequent analysis.

• Lectin Array Incubation

The prepared and labeled samples are incubated on our proprietary lectin microarrays. The glycoproteins bind to their specific lectin probes on the array. Our comprehensive panel of lectins ensures a wide-ranging analysis of different glycan structures present in your sample.

• Signal Detection and Data Acquisition

After incubation and washing, the microarray slides are scanned with a high-sensitivity scanner to detect the fluorescent signals from the labeled glycoproteins. The intensity of each spot's signal correlates directly with the amount of a specific glycoprotein-lectin interaction, providing quantitative data.

• Bioinformatics and Data Analysis

The raw data is then processed through our advanced bioinformatics pipeline. Our experts analyze the data to identify and quantify differences in glycosylation patterns between sample groups, providing you with a comprehensive report.

Publication Data

Advantages

• Our microarray platform allows for the simultaneous analysis of thousands of binding events, which is crucial for the rapid screening of a large number of samples. This is a major improvement over time-consuming, low-throughput methods.
• Our microarrays are optimized for high sensitivity, requiring only small sample volumes, making them suitable for precious biological samples. The reproducible nature of our microarray platforms is a key advantage, ensuring consistent and comparable data across experiments.
• By combining microarray technology with complementary methods like mass spectrometry, we provide both a broad glycan profile and detailed structural information, a combination essential for in-depth scientific discovery.

Application

• Lectin microarrays detect disease-associated glycan alterations (e.g., in cancer, liver fibrosis, and Alzheimer's disease), enabling the discovery of non-invasive biomarkers for early diagnosis and monitoring of pathological progression
• The technology profiles glycans from varied sources, including bacteria, viruses, extracellular vesicles, and clinical samples, uncovering roles in host-pathogen interactions, particle assembly, and disease mechanisms.
• This technology identifies functionally significant cell surface glycan signatures, revealing differences between cell types (e.g., normal vs. cancerous) and tracking glycosylation changes during processes like immune cell differentiation and pathogen-host interactions

Frequently Asked Questions

Associated Services

Lectin binding protein microarray services decode glycan-binding specificities across diverse biological systems—revealing critical interactions in pathogen adhesion, immune regulation, and cancer biomarkers through high-throughput screening of immobilized lectins. To extend this static profiling into dynamic functional studies of glycan-mediated interactions under native membrane conditions, we offer Liquid Glycan Display Array (LiGA). This platform engineers fluidic membranes to mimic physiological cell-surface environments, enabling:

CD BioGlyco provides high-quality microarray tools and convenient detection services for studying lectin-related carbohydrate structure and glycoprotein analysis. We have a large and well-equipped team of scientists who are committed to cooperating with researchers from all over the world. By choosing outsourcing testing services, your advantage is that you do not need to consider any other expenses, such as equipment, laboratory space, supervision or management. This convenient method can quickly turn around and provide reliable results, thus saving valuable time and resources. Please contact us for more detailed information.

Reference

1. Sun, Y.; et al. A human lectin microarray for sperm surface glycosylation analysis. Molecular & Cellular Proteomics. 2016, 15(9): 2839-2851. (Open Access)