Protein-saccharide interactions, exemplified by glycoprotein receptors and saccharides, play pivotal roles in myriad cellular recognitions, encompassing cell growth regulation, differentiation, adhesion, cancer cell metastasis, cellular trafficking, inflammation induced by bacteria and viruses, and immune responses. CD BioGlyco provides the GlycoNano™ Platform for the development of saccharide-functionalized materials within the glycobiology-related research domain. As a notable illustration, Magnetic Glyconanoparticles, such as magnetite, have demonstrated efficacy in rapid pathogen detection, decontamination, and strain differentiation. This efficacy is directly attributable to the lectin-saccharide interaction between the glycoproteins and glycolipids expressed on the cell surfaces of pathogens and the mono- or disaccharides-functionalized capturing modules.
We first dissolve FeCl3·6H2O and FeSO4·7H2O separately in deionized water at room temperature. Then, combine the two solutions and heat them with magnetic stirring. Afterward, we add NaOH to initiate the co-precipitation reaction. The resulting magnetic nanoparticles are washed multiple times with deionized water to achieve a neutral pH.
Galactose is added to the uncoated magnetic nanoparticles and the mixture is stirred to allow surface coating. The coated magnetic nanoparticles are separated magnetically and washed with deionized water to maintain a neutral pH.
Diluted samples are analyzed using a zeta potential analyzer to determine particle size distribution and surface charge. We employ atomic force microscopy (AFM) to evaluate the shape and height of the magnetic nanoparticles. Raman microscopy is used to acquire spectra for further characterization of the magnetic nanoparticles.
We prepare Benedict's solution and use it to test the presence of reducing sugars on the surfaces of the magnetic nanoparticle. Supernatants from the magnetic nanoparticle are mixed with Benedict's solution and heated to observe color changes indicative of reducing sugars as indicative of the presence of reducing sugars.
Technology: Fourier transform infrared spectroscopy (FTIR), Transmission electron microscopy (TEM), electrophoretic light scattering spectrophotometer (ELS), X-ray diffractometer (XRD), Magnetic resonance (MR)
Journal: 10.3390/catal9010007
IF: 3.8
Published: 2016
Results: In pursuit of developing superparamagnetic iron oxide nanoparticles (SPIONs) with specialized functionalities, capable of stably dispersing in physiological conditions and specifically targeting the liver, the authors herein encapsulated SPIONs with polyvinylbenzyl-O-β-D-galactopyranosyl-D-gluconamide (PVLA), adorned with galactose moieties that are recognized by asialoglycoprotein receptors (ASGP-R) on hepatocytes. As a control, SPIONs coordinated with 2-pyrrolidone were prepared. Characterization of particle dimensions, size distribution, structural integrity, and coating was performed via TEM, ELS, XRD, and FT-IR spectroscopy, respectively. Intracellular uptake of PVLA-coated SPIONs was visualized using confocal laser scanning microscopy, with hepatocyte-specific delivery validated through MR imaging of rat liver. MRI results revealed enhanced liver accumulation specificity of PVLA-coated SPIONs versus controls, suggesting their potential as liver-targeted MRI contrast agents. This study thus demonstrates the efficacy of galactose-modified SPIONs in achieving specific liver targeting and their stable integration within biological environments, thereby offering promising prospects for nanomedical applications in liver cancer diagnosis and treatment.
CD BioGlyco's comprehensive and one-stop service ensures the synthesis, functionalization, and thorough characterization of galactose-coated magnetic nanoparticles for potential applications in various fields such as biomedicine and material science. If you want to learn more about our Glyconanoparticle Development Service, contact us!
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