Oligonucleotide-based Bases Modification Service

Applications of Modified Oligonucleotides

Oligonucleotides may modulate gene or miRNA expression by RNAi, target degradation, non-coding RNA inhibition, and gene activation. However, unmodified RNA oligonucleotides are always rapidly digested in vitro and in vivo by endogenous ribonucleases, a process that requires the 2'-OH group to hydrolyze the RNA. Therefore, ribose modification at the 2' position has been widely used to protect oligonucleotides from nuclease attack and increase binding affinity to the target. These modifications are introduced at base positions in oligonucleotide molecules by chemical synthesis. Chemically modified oligonucleotides are used to optimize nucleic acid drugs, containing increasing specificity, affinity, and stability.

Oligonucleotide-based Bases Modification Service at CD BioGlyco

CD BioGlyco offers a variety of oligonucleotide modifications introduced in the process of oligo synthesis, and post-synthetically, according to custom requirements, which may increase RNA-binding affinity, the nuclease stability, and pharmacokinetic features of oligonucleotides for therapeutic applications. We offer diverse oligonucleotide-based base modification services.

• Affinity-plus base
• 2'-O-methoxy-ethyl bases (2'-MOE)
• 2'-O-methyl RNA bases
• Inosine
• Fluoro bases
• Others: Deoxyuridine, deoxyinosine, 2-aminopurine, 2,6-diaminopurine, 5-bromo dU, 5-methyl dC, 5-nitroindole.

Oligonucleotide Synthesis Service at CD BioGlyco

CD BioGlyco tailors the process of oligonucleotide synthesis concerning the required length, sequence, and modifications according to custom research needs. The process of custom synthesis generally involves several steps, including the design and optimization of synthetic routes, chemical synthesis, purification and refinement, analysis, and quality determination of the synthesized compounds to ensure to requirement t qualified products. CD BioGlyco customizes this process accordingly to cater to the customer's research needs.

Fig.1 Oligonucleotide-based modification and synthesis. (CD BioGlyco)

Applications

• Molecular diagnostics: Sensitive and specific detection of specific sequences can be achieved by introducing fluorescent probes, biotin, or other labels on oligonucleotides.
• Molecular engineering: Oligonucleotide base modification may be applied in molecular engineering applications, for example constructing DNA/RNA nanostructures, synthesizing artificial genetic systems, and designing sequences with specific functions. Base modification may regulate the properties and interactions of oligonucleotides, expanding their application potential in nanotechnology and biotechnology.
• Biomedical research: The affinity, stability, and metabolic properties of oligonucleotides may be changed by introducing specific base modifications, to achieve precise regulation and research on biological processes.
• Drug development: Oligonucleotide base modification may be applied in various stages of drug development, including drug target identification, drug delivery system design, and drug safety assessment. The reason is that base modification can change the pharmacokinetic properties, cell permeability, and specificity of oligonucleotides.

Advantages

• Professional team: High-level scientists apply the latest methods and tools to provide flexible customized oligonucleotide-based modification and synthesis services according to the needs of customers.
• First-class experimental conditions: We own advanced laboratory equipment that can provide accurate and reliable experimental results and products.
• Strict quality control: To provide high-quality oligonucleotides, all products are produced under strict quality control procedures at CD BioGlyco.

CD BioGlyco provides comprehensive high-quality oligonucleotide-based modification and synthesis services. In addition, we also provide Custom Sugar-nucleotides Synthesis and Custom Carbohydrate Synthesis. Please feel free to contact us if you need to consult our modification and synthesis services.