Ceb, as a type of sphingolipid, contains a sugar head group (galactose, glucose) covalently attached to a two-tailed ceramide via a glycosidic linkage, through the primary hydroxyl group, and an additional amide-bonded fatty acid acyl chain attached to the sphingoid backbone. Ceb is synthesized in an organism catalyzed by membrane-bound enzymes on the endoplasmic reticulum leaflet body, and metabolism is decomposed in lysosomes. Ceb not only serves as an important component of the plasma membrane to maintain the basic structural skeleton of the cell, but also plays a major role in the physiological and biochemical processes such as cell growth, proliferation, and aging. In addition to medical research, Cebs are also widely used in whitening and moisturizing cosmetics such as research and development.
Fig.1 General structures of glycosphingosines and cerebrosides. (Chao & McLuckey, 2021)
Given the critical importance of the specific biological roles of Cebs in health and disease states, it is essential to distinguish between the multiple isomers. Based on the Cell Surface Glycolipid Glycoengineering Service, CD BioGlyco offers the following services:
We provide cerebroside modification service on cell surfaces by specific gene editing and expression. We mainly regulate the expression of galactosyltransferase and sulfotransferase, and then achieve the purpose of modification. Moreover, we offer a sulphation modification service.
CD BioGlyco has a complete process applied to sample extraction isolation purification and structural characterization of Ceb. We offer organic solvent extraction, purification by passing through a column, or purification service using multi-dimensional liquid chromatography followed by analysis by nuclear magnetic resonance (NMR) or liquid chromatograph mass spectrometer (LC-MS).
CD BioGlyco uses naturally sourced lipids and provides differential scanning calorimetry, confocal microscopy, and atomic force microscopy services to analyze samples to characterize lipid domains in supported planar bilayers and giant unilamellar vesicles.
CD BioGlyco provides new strategies combining shotgun tandem mass spectrometry with gas-phase ion chemistry for the differentiation and quantification of ceruloplasmin and glycosphingosine.
Fig.2 Schematic of Cebs-based cell surface glycoengineering service. (CD BioGlyco)
Paper Title: In-depth structural characterization and quantification of cerebrosides and glycosphingosines with gas-phase ion chemistry
Technology: Combined shotgun tandem MS coupled with gas-phase ionic chemistry
Journal: Analytical Chemistry
IF: 8.008
Published: 2021
Results: In this work, we demonstrate a shotgun tandem mass spectrometric approach including gas phase ion/ion chemistry and ion trap collision-induced dissociation (CID) to provide in-depth structural information from arabinoside and glycosphingosine. The subsequent CID of these cations allows each isomer to be identified and analyzed. This strategy distinguishes between monosaccharide headgroup diastereomers and hetero-glycoside junctions to the sheath chain backbone. Furthermore, the relative quantification of the three heterogeneous encephalosines and the four heterogeneous sugar sphingosine mixtures was achieved by analyzing the normalized percentage area of the diagnostic product ions. Analytical performance in precision, repeatability, and daytime precision.
Fig.3 Identification of double-bond position from the monounsaturated fatty acyl side chain on cerebrosides. (Chao & McLuckey, 2021)
CD BioGlyco aspires to be your preferred and trusted assistant that provides Cell Surface Glycoengineering services. We have advanced technology and experienced staff dedicated to advancing your project and reducing overall development time. Moreover, we provide high-quality Glycosphingolipid, Ganglioside, Globoside, and Lipid Raft-based Cell Surface Glycoengineering Services. If you are interested in our services, please feel free to contact us.
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