Applications of Base-based Halogenation Modification

Typically, nucleotide modification is achieved by halogenation at the base of a nucleoside or nucleotide to change its chemical structure and properties. In this step, halogen atoms, such as bromine or iodine, are introduced onto the bases of nucleosides or nucleotides to form halogenated groups. Such modifications change the charge distribution, chemical properties, and spatial distribution of the bases. A common method is to use a specific reagent, such as iodic acid or trichloroacetic acid, to react with the base to form a halogenated compound. Base-based halogenation modification has a wide range of applications in DNA sequencing, DNA methylation analysis, and DNA modification studies. Modifying the properties of these molecules can help scientists better understand the functional mechanisms of these molecules in biological processes such as gene expression and protein synthesis. In addition, it can be used to explore and study the role of disease-related genes and to develop novel drugs.

Base-based Halogenation Modification Service at CD BioGlyco

CD BioGlyco has established a Glyco™ Synthesis Platform to provide Custom Sugar-nucleotides Synthesis services to our clients. We provide not only an Oligonucleotide Modification Service but also Nucleoside & Nucleotide Modification Service. We have advanced synthesis instruments and experienced technicians to ensure the quality of base-based halogenation modification. The details of our experiments are as follows:

• The first step is the preparation of nucleosides or nucleotides containing bases. To protect specific functional groups, a protecting group is introduced at the base of the nucleotide before halogenation modification to prevent it from being unnecessarily interfered with in subsequent reactions.
• Subsequently, we perform reactions of halogen compounds with starting materials in appropriate solvents and at lower temperatures to introduce halogens to specific sites in the nucleotide. Our commonly used halogen compounds include bromoacetyl and sulfonyl bromide. Then we control the stereoselectivity of halogen substitution according to your specific requirements. This is usually achieved by introducing chiral ligands or selective catalysts.
• Finally, the protecting groups were removed and the products were purified and characterized.

Fig.1 Flow chart of base-based halogenation modification. (CD BioGlyco)

Applications

• Nucleosides & nucleotide modifications are widely used in modern genomics and transcriptomics research, providing essential tools for understanding biological processes and studying diseases.
• Nucleotide modification technology provides a new way for the design and development of antiviral drugs and is a powerful tool for research on viral infections.
• Modified nucleotides can be used as probes for specific sequences for gene detection and mutation analysis. 

Advantages of Us

• We have a stock of high-quality raw materials that are strictly screened and verified to meet international standards and quality requirements.
• We work closely with our clients to understand their specific requirements and provide a one-to-one customized service.
• Our technicians have a wide range of knowledge and expertise, with extensive hands-on experience in a variety of fields.

CD BioGlyco focuses on providing high-quality base-based halogenation modification services for nucleosides and nucleotides. Through precise synthetic processes and advanced technology, we selectively achieve base halogenation modification and provide custom solutions for our clients. If you are interested in our services, please feel free to contact us for more details.